ARG81853
Mouse CD13 ELISA Kit
Mouse CD13 ELISA 试剂盒 for ELISA and Mouse
内含物
| 货号 | 内含物名称 | 包装 | 温度 |
|---|---|---|---|
| ARG81853-001 | Antibody-coated microplate | 8 X 12 strips | 4°C. Unused strips should be sealed tightly in the air-tight pouch. |
| ARG81853-002 | Standard | 2 X 20 ng/vial | 4°C |
| ARG81853-003 | Standard/Sample diluent | 30 ml (Ready to use) | 4°C |
| ARG81853-004 | Antibody conjugate concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG81853-005 | Antibody diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG81853-006 | HRP-Streptavidin concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG81853-007 | HRP-Streptavidin diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG81853-008 | 25X Wash buffer | 20 ml | 4°C |
| ARG81853-009 | TMB substrate | 10 ml (Ready to use) | 4°C (Protect from light) |
| ARG81853-010 | STOP solution | 10 ml (Ready to use) | 4°C |
| ARG81853-011 | Plate sealer | 4 strips | Room temperature |
概述
| 产品描述 | ARG81853 Mouse CD13 ELISA Kit is an Enzyme Immunoassay kit for the quantification of Mouse CD13 in serum, plasma (heparin, EDTA) and cell culture supernatants. |
|---|---|
| 反应物种 | Ms |
| 应用 | ELISA |
| 特异性 | There is no detectable cross-reactivity with other relevant proteins. |
| 靶点名称 | CD13 |
| 偶联标记 | HRP |
| 偶联标记说明 | Substrate: TMB and read at 450 nm. |
| 灵敏度 | 156 pg/ml |
| 样品类型 | Serum, plasma (heparin, EDTA) and cell culture supernatants. |
| 标准范围 | 312 - 20000 pg/ml |
| 样本量 | 100 µl |
| 精确 | Intra-Assay CV: 7.1%; Inter-Assay CV: 7.8% |
| 別名 | AP-N; PEPN; LAP1; CD antigen CD13; Aminopeptidase M; gp150; Aminopeptidase N; EC 3.4.11.2; Myeloid plasma membrane glycoprotein CD13; APN; CD13; P150; AP-M; GP150; hAPN; Microsomal aminopeptidase; Alanyl aminopeptidase |
应用说明
| 检测时间 | ~ 5 hours |
|---|
属性
| 形式 | 96 well |
|---|---|
| 存放说明 | Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | ANPEP |
| 全名 | alanyl (membrane) aminopeptidase |
| 背景介绍 | Aminopeptidase N is located in the small-intestinal and renal microvillar membrane, and also in other plasma membranes. In the small intestine aminopeptidase N plays a role in the final digestion of peptides generated from hydrolysis of proteins by gastric and pancreatic proteases. Its function in proximal tubular epithelial cells and other cell types is less clear. The large extracellular carboxyterminal domain contains a pentapeptide consensus sequence characteristic of members of the zinc-binding metalloproteinase superfamily. Sequence comparisons with known enzymes of this class showed that CD13 and aminopeptidase N are identical. The latter enzyme was thought to be involved in the metabolism of regulatory peptides by diverse cell types, including small intestinal and renal tubular epithelial cells, macrophages, granulocytes, and synaptic membranes from the CNS. Human aminopeptidase N is a receptor for one strain of human coronavirus that is an important cause of upper respiratory tract infections. Defects in this gene appear to be a cause of various types of leukemia or lymphoma. [provided by RefSeq, Jul 2008] |
| 生物功能 | Broad specificity aminopeptidase. Plays a role in the final digestion of peptides generated from hydrolysis of proteins by gastric and pancreatic proteases. May play a critical role in the pathogenesis of cholesterol gallstone disease. May be involved in the metabolism of regulatory peptides of diverse cell types, responsible for the processing of peptide hormones, such as angiotensin III and IV, neuropeptides, and chemokines. Found to cleave antigen peptides bound to major histocompatibility complex class II molecules of presenting cells and to degrade neurotransmitters at synaptic junctions. Is also implicated as a regulator of IL-8 bioavailability in the endometrium, and therefore may contribute to the regulation of angiogenesis. Is used as a marker for acute myeloid leukemia and plays a role in tumor invasion. In case of human coronavirus 229E (HCoV-229E) infection, serves as receptor for HCoV-229E spike glycoprotein. Mediates as well human cytomegalovirus (HCMV) infection. [UniProt] |
| 产品亮点 | Related products: CD13 antibodies; CD13 ELISA Kits; CD13 recombinant proteins; New ELISA data calculation tool: Simplify the ELISA analysis by GainData |
| 翻译后修饰 | Sulfated. N- and O-glycosylated. May undergo proteolysis and give rise to a soluble form. [UniProt] |
检测图片 (1) Click the Picture to Zoom In
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ARG81853 Mouse CD13 ELISA Kit standard curve image
ARG81853 Mouse CD13 ELISA Kit results of a typical standard run with optical density reading at 450 nm.
| Title | Download Link |
|---|---|
| ARG81853 Mouse CD13 ELISA Kit User's manual |
 Download
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