ARG82634
Mouse CD253 / TRAIL ELISA Kit
Mouse CD253 / TRAIL ELISA 试剂盒 for ELISA and Mouse
内含物
| 货号 | 内含物名称 | 包装 | 温度 |
|---|---|---|---|
| ARG82634-001 | Antibody-coated microplate | 8 X 12 strips | 4°C. Unused strips should be sealed tightly in the air-tight pouch. |
| ARG82634-002 | Standard (Lyophilized) | 2 X 10 ng/vial | 4°C |
| ARG82634-003 | Standard/Sample diluent | 30 ml | 4°C |
| ARG82634-004 | Antibody conjugate concentrate (100X) | 1 vial (120 µl) | 4°C |
| ARG82634-005 | Antibody diluent buffer | 12 ml | 4°C |
| ARG82634-006 | HRP-Streptavidin concentrate (100X) | 1 vial (120 µl) | 4°C |
| ARG82634-007 | HRP-Streptavidin diluent buffer | 12 ml | 4°C |
| ARG82634-008 | Wash Buffer (20X) | 30 ml | 4°C |
| ARG82634-009 | TMB substrate | 12 ml | 4°C (Protect from light) |
| ARG82634-010 | STOP solution | 12 ml | 4°C |
| ARG82634-011 | Plate sealer | 3 strips | Room temperature |
概述
| 产品描述 | ARG82634 Mouse CD253 / TRAIL ELISA Kit is an Enzyme Immunoassay kit for the quantification of Mouse CD253 / TRAIL in serum, plasma and cell culture supernatants. |
|---|---|
| 反应物种 | Ms |
| 应用 | ELISA |
| 特异性 | No detectable cross-reactivity with other relevant proteins. |
| 靶点名称 | CD253 / TRAIL |
| 偶联标记 | HRP |
| 偶联标记说明 | Substrate: TMB and read at 450 nm. |
| 灵敏度 | 7.8 pg/ml |
| 样品类型 | Serum, plasma and cell culture supernatants. |
| 标准范围 | 15.6 - 1000 pg/ml |
| 样本量 | 100 µl |
| 別名 | TL2; CD253; Protein TRAIL; TNF-related apoptosis-inducing ligand; TRAIL; CD antigen CD253; Apo-2 ligand; Apo-2L; APO2L; Tumor necrosis factor ligand superfamily member 10 |
应用说明
| 检测时间 | ~ 4 hours |
|---|
属性
| 形式 | 96 well |
|---|---|
| 存放说明 | Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 |
Swiss-port # P50592 Mouse Tumor necrosis factor ligand superfamily member 10 |
|---|---|
| 基因名称 | TNFSF10 |
| 全名 | tumor necrosis factor (ligand) superfamily, member 10 |
| 背景介绍 | The protein encoded by this gene is a cytokine that belongs to the tumor necrosis factor (TNF) ligand family. This protein preferentially induces apoptosis in transformed and tumor cells, but does not appear to kill normal cells although it is expressed at a significant level in most normal tissues. This protein binds to several members of TNF receptor superfamily including TNFRSF10A/TRAILR1, TNFRSF10B/TRAILR2, TNFRSF10C/TRAILR3, TNFRSF10D/TRAILR4, and possibly also to TNFRSF11B/OPG. The activity of this protein may be modulated by binding to the decoy receptors TNFRSF10C/TRAILR3, TNFRSF10D/TRAILR4, and TNFRSF11B/OPG that cannot induce apoptosis. The binding of this protein to its receptors has been shown to trigger the activation of MAPK8/JNK, caspase 8, and caspase 3. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2010] |
| 生物功能 | Cytokine that binds to TNFRSF10A/TRAILR1, TNFRSF10B/TRAILR2, TNFRSF10C/TRAILR3, TNFRSF10D/TRAILR4 and possibly also to TNFRSF11B/OPG. Induces apoptosis. Its activity may be modulated by binding to the decoy receptors TNFRSF10C/TRAILR3, TNFRSF10D/TRAILR4 and TNFRSF11B/OPG that cannot induce apoptosis. [UniProt] |
| 细胞定位 | Membrane; Single-pass type II membrane protein. [UniProt] |
| 产品亮点 | Related products: CD253 antibodies; CD253 ELISA Kits; New ELISA data calculation tool: Simplify the ELISA analysis by GainData |
| 翻译后修饰 | Tyrosine phosphorylated by PKDCC/VLK. [UniProt] |
检测图片 (1) Click the Picture to Zoom In
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ARG82634 Mouse CD253 / TRAIL ELISA Kit standard curve image
ARG82634 Mouse CD253 / TRAIL ELISA Kit results of a typical standard run with optical density reading at 450 nm.
| Title | Download Link |
|---|---|
| ARG82634 Mouse CD253 / TRAIL ELISA Kit User manual |
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