ARG81712
Mouse CD26 / DPP4 ELISA Kit
Mouse CD26 / DPP4 ELISA 试剂盒 for ELISA and Mouse
内含物
| 货号 | 内含物名称 | 包装 | 温度 |
|---|---|---|---|
| ARG81712-001 | Antibody-coated microplate | 8 X 12 strips | 4°C. Unused strips should be sealed tightly in the air-tight pouch. |
| ARG81712-002 | Standard | 2 X 10 ng/vial | 4°C |
| ARG81712-003 | Standard/Sample diluent | 30 ml (Ready to use) | 4°C |
| ARG81712-004 | Antibody conjugate concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG81712-005 | Antibody diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG81712-006 | HRP-Streptavidin concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG81712-007 | HRP-Streptavidin diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG81712-008 | 25X Wash buffer | 20 ml | 4°C |
| ARG81712-009 | TMB substrate | 10 ml (Ready to use) | 4°C (Protect from light) |
| ARG81712-010 | STOP solution | 10 ml (Ready to use) | 4°C |
| ARG81712-011 | Plate sealer | 4 strips | Room temperature |
概述
| 产品描述 | ARG81712 Mouse CD26 / DPP4 ELISA Kit is an Enzyme Immunoassay kit for the quantification of Mouse CD26 / DPP4 in serum, plasma (heparin, EDTA) and cell culture supernatants. |
|---|---|
| 反应物种 | Ms |
| 应用 | ELISA |
| 特异性 | There is no detectable cross-reactivity with other relevant proteins. |
| 靶点名称 | CD26 / DPP4 |
| 偶联标记 | HRP |
| 偶联标记说明 | Substrate: TMB and read at 450 nm. |
| 灵敏度 | 31.25 pg/ml |
| 样品类型 | Serum, plasma (heparin, EDTA) and cell culture supernatants. |
| 标准范围 | 62.5 - 4000 pg/ml |
| 样本量 | 100 µl |
| 精确 | Intra-Assay CV: 6.5%; Inter-Assay CV: 7.2% |
| 別名 | T-cell activation antigen CD26; ADCP2; ADCP-2; DPP IV; Adenosine deaminase complexing protein 2; CD26; EC 3.4.14.5; ADABP; Dipeptidyl peptidase IV soluble form; Dipeptidyl peptidase IV; Dipeptidyl peptidase 4; Dipeptidyl peptidase IV membrane form; TP103; DPPIV; CD antigen CD26 |
应用说明
| 检测时间 | ~ 5 hours |
|---|
属性
| 形式 | 96 well |
|---|---|
| 存放说明 | Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | DPP4 |
| 全名 | dipeptidyl-peptidase 4 |
| 背景介绍 | The protein encoded by this gene is identical to adenosine deaminase complexing protein-2, and to the T-cell activation antigen CD26. It is an intrinsic membrane glycoprotein and a serine exopeptidase that cleaves X-proline dipeptides from the N-terminus of polypeptides. [provided by RefSeq, Jul 2008] |
| 生物功能 | Cell surface glycoprotein receptor involved in the costimulatory signal essential for T-cell receptor (TCR)-mediated T-cell activation. Acts as a positive regulator of T-cell coactivation, by binding at least ADA, CAV1, IGF2R, and PTPRC. Its binding to CAV1 and CARD11 induces T-cell proliferation and NF-kappa-B activation in a T-cell receptor/CD3-dependent manner. Its interaction with ADA also regulates lymphocyte-epithelial cell adhesion. In association with FAP is involved in the pericellular proteolysis of the extracellular matrix (ECM), the migration and invasion of endothelial cells into the ECM. May be involved in the promotion of lymphatic endothelial cells adhesion, migration and tube formation. When overexpressed, enhanced cell proliferation, a process inhibited by GPC3. Acts also as a serine exopeptidase with a dipeptidyl peptidase activity that regulates various physiological processes by cleaving peptides in the circulation, including many chemokines, mitogenic growth factors, neuropeptides and peptide hormones. Removes N-terminal dipeptides sequentially from polypeptides having unsubstituted N-termini provided that the penultimate residue is proline. [UniProt] |
| 产品亮点 | Related products: CD26 antibodies; CD26 ELISA Kits; New ELISA data calculation tool: Simplify the ELISA analysis by GainData |
| 翻译后修饰 | The soluble form (Dipeptidyl peptidase 4 soluble form also named SDPP) derives from the membrane form (Dipeptidyl peptidase 4 membrane form also named MDPP) by proteolytic processing. N- and O-Glycosylated. Phosphorylated. Mannose 6-phosphate residues in the carbohydrate moiety are necessary for interaction with IGF2R in activated T-cells. Mannose 6-phosphorylation is induced during T-cell activation. [UniProt] |
检测图片 (1) Click the Picture to Zoom In
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ARG81712 Mouse CD26 / DPP4 ELISA Kit standard curve image
ARG81712 Mouse CD26 / DPP4 ELISA Kit results of a typical standard run with optical density reading at 450 nm.
| Title | Download Link |
|---|---|
| ARG81712 Mouse CD26 / DPP4 ELISA Kit User's manual |
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