ARG81930

Mouse CD274 / PD-L1 ELISA Kit

Datasheet

参考文献1

订购信息

包装

价格 (USD)

96 wells

655

arigo in CHINA:
恩瑞科生物
☎ 400-886-0853,
0571-8708-1890

内含物

货号	内含物名称	包装	温度
ARG81930-001	Antibody-coated microplate	8 X 12 strips	4°C. Unused strips should be sealed tightly in the air-tight pouch.
ARG81930-002	Standard	2 X 10 ng/vial	4°C
ARG81930-003	Standard/Sample diluent	30 ml (Ready to use)	4°C
ARG81930-004	Antibody conjugate concentrate (100X)	1 vial (100 µl)	4°C
ARG81930-005	Antibody diluent buffer	12 ml (Ready to use)	4°C
ARG81930-006	HRP-Streptavidin concentrate (100X)	1 vial (100 µl)	4°C
ARG81930-007	HRP-Streptavidin diluent buffer	12 ml (Ready to use)	4°C
ARG81930-008	25X Wash buffer	20 ml	4°C
ARG81930-009	TMB substrate	10 ml (Ready to use)	4°C (Protect from light)
ARG81930-010	STOP solution	10 ml (Ready to use)	4°C
ARG81930-011	Plate sealer	4 strips	Room temperature

概述

产品描述	ARG81930 Mouse CD274 / PD-L1 ELISA Kit is an Enzyme Immunoassay kit for the quantification of Mouse CD274 / PD-L1 in serum, plasma (heparin, EDTA) and cell culture supernatants.
反应物种	Ms
应用	ELISA
特异性	There is no detectable cross-reactivity with other relevant proteins.
靶点名称	CD274 / PD-L1
偶联标记	HRP
偶联标记说明	Substrate: TMB and read at 450 nm.
灵敏度	15.6 pg/ml
样品类型	Serum, plasma (heparin, EDTA) and cell culture supernatants.
标准范围	31.2 - 2000 pg/ml
样本量	100 µl
精确	Intra-Assay CV: 6.7%; Inter-Assay CV: 7.5%
別名	Programmed cell death 1 ligand 1; B7-H1; B7H1; PDL1; PDCD1 ligand 1; B7 homolog 1; PD-L1; CD antigen CD274; PDCD1L1; B7-H; Programmed death ligand 1; PDCD1LG1

应用说明

检测时间	~ 5 hours

属性

形式	96 well
存放说明	Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components.
注意事项	For laboratory research only, not for drug, diagnostic or other use.

生物信息

数据库连接	GeneID: 60533 Mouse CD274 Swiss-port # Q9EP73 Mouse Programmed cell death 1 ligand 1
基因名称	CD274
全名	CD274 molecule
背景介绍	CD274 / PD-L1 is an immune inhibitory receptor ligand. It is expressed by hematopoietic and non-hematopoietic cells, such as T cells and B cells and various types of tumor cells. The encoded protein is a type I transmembrane protein that has immunoglobulin V-like and C-like domains. Interaction of this ligand with its receptor inhibits T-cell activation and cytokine production. During infection or inflammation of normal tissue, this interaction is important for preventing autoimmunity by maintaining homeostasis of the immune response. In tumor microenvironments, this interaction provides an immune escape for tumor cells through cytotoxic T-cell inactivation. Expression of this gene in tumor cells is considered to be prognostic in many types of human malignancies, including colon cancer and renal cell carcinoma. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Sep 2015]
生物功能	CD274 / PD-L1 plays a critical role in induction and maintenance of immune tolerance to self (PubMed:11015443, PubMed:28813417, PubMed:28813410). As a ligand for the inhibitory receptor PDCD1/PD-1, modulates the activation threshold of T-cells and limits T-cell effector response (PubMed:11015443, PubMed:28813417, PubMed:28813410). Through a yet unknown activating receptor, may costimulate T-cell subsets that predominantly produce interleukin-10 (IL10) (PubMed:10581077). The PDCD1-mediated inhibitory pathway is exploited by tumors to attenuate anti-tumor immunity and escape destruction by the immune system, thereby facilitating tumor survival (PubMed:28813417, PubMed:28813410). The interaction with PDCD1/PD-1 inhibits cytotoxic T lymphocytes (CTLs) effector function. The blockage of the PDCD1-mediated pathway results in the reversal of the exhausted T-cell phenotype and the normalization of the anti-tumor response, providing a rationale for cancer immunotherapy. [UniProt]
产品亮点	Related products: PD-L1 antibodies; PD-L1 ELISA Kits; Related news: Detecting exosomal PD-L1 secreted by cancer cells The best solution for PD-1/PD-L1 research Examining CTL/NK-mediated cytotoxicity by ELISA New ELISA data calculation tool: Simplify the ELISA analysis by GainData

检测图片 (1) Click the Picture to Zoom In

ARG81930 Mouse CD274 / PD-L1 ELISA Kit standard curve image

ARG81930 Mouse CD274 / PD-L1 ELISA Kit results of a typical standard run with optical density reading at 450 nm.

文献引用

WEE1 inhibitor and ataxia telangiectasia and RAD3-related inhibitor trigger stimulator of interferon gene-dependent immune response and enhance tumor treatment efficacy through programmed death-ligand 1 blockade

ELISA / Mouse / cell culture supernatant

Xue Wu et al.
Cancer Sci., (2021)