ARG81716
Mouse CD36 ELISA Kit
Mouse CD36 ELISA 试剂盒 for ELISA and Mouse
内含物
| 货号 | 内含物名称 | 包装 | 温度 |
|---|---|---|---|
| ARG81716-001 | Antibody-coated microplate | 8 X 12 strips | 4°C. Unused strips should be sealed tightly in the air-tight pouch. |
| ARG81716-002 | Standard | 2 X 10 ng/vial | 4°C |
| ARG81716-003 | Standard/Sample diluent | 30 ml (Ready to use) | 4°C |
| ARG81716-004 | Antibody conjugate concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG81716-005 | Antibody diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG81716-006 | HRP-Streptavidin concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG81716-007 | HRP-Streptavidin diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG81716-008 | 25X Wash buffer | 20 ml | 4°C |
| ARG81716-009 | TMB substrate | 10 ml (Ready to use) | 4°C (Protect from light) |
| ARG81716-010 | STOP solution | 10 ml (Ready to use) | 4°C |
| ARG81716-011 | Plate sealer | 4 strips | Room temperature |
概述
| 产品描述 | ARG81716 Mouse CD36 ELISA Kit is an Enzyme Immunoassay kit for the quantification of Mouse CD36 in serum, plasma (heparin, EDTA) and cell culture supernatants. |
|---|---|
| 反应物种 | Ms |
| 应用 | ELISA |
| 特异性 | There is no detectable cross-reactivity with other relevant proteins. |
| 靶点名称 | CD36 |
| 偶联标记 | HRP |
| 偶联标记说明 | Substrate: TMB and read at 450 nm. |
| 灵敏度 | 78 pg/ml |
| 样品类型 | Serum, plasma (heparin, EDTA) and cell culture supernatants. |
| 标准范围 | 156 - 10000 pg/ml |
| 样本量 | 100 µl |
| 精确 | Intra-Assay CV: 5.5%; Inter-Assay CV: 6.5% |
| 別名 | GPIV; CHDS7; Platelet glycoprotein 4; CD antigen CD36; PAS-4; PASIV; Glycoprotein IIIb; PAS IV; GPIIIB; FAT; SCARB3; GP3B; Leukocyte differentiation antigen CD36; Platelet collagen receptor; BDPLT10; Thrombospondin receptor; GP4; Fatty acid translocase; Platelet glycoprotein IV |
应用说明
| 检测时间 | ~ 5 hours |
|---|
属性
| 形式 | 96 well |
|---|---|
| 存放说明 | Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | CD36 |
| 全名 | CD36 molecule (thrombospondin receptor) |
| 背景介绍 | The protein encoded by this gene is the fourth major glycoprotein of the platelet surface and serves as a receptor for thrombospondin in platelets and various cell lines. Since thrombospondins are widely distributed proteins involved in a variety of adhesive processes, this protein may have important functions as a cell adhesion molecule. It binds to collagen, thrombospondin, anionic phospholipids and oxidized LDL. It directly mediates cytoadherence of Plasmodium falciparum parasitized erythrocytes and it binds long chain fatty acids and may function in the transport and/or as a regulator of fatty acid transport. Mutations in this gene cause platelet glycoprotein deficiency. Multiple alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Feb 2014] |
| 生物功能 | Binds to collagen, thrombospondin, anionic phospholipids and oxidized low-density lipoprotein (oxLDL). May function as a cell adhesion molecule. Directly mediates cytoadherence of Plasmodium falciparum parasitized erythrocytes. Binds long chain fatty acids and may function in the transport and/or as a regulator of fatty acid transport. Receptor for thombospondins, THBS1 AND THBS2, mediating their antiangiogenic effects. As a coreceptor for TLR4-TLR6 heterodimer, promotes inflammation in monocytes/macrophages. Upon ligand binding, such as oxLDL or amyloid-beta 42, rapidly induces the formation of a heterodimer of TLR4 and TLR6, which is internalized and triggers inflammatory response, leading to NF-kappa-B-dependent production of CXCL1, CXCL2 and CCL9 cytokines, via MYD88 signaling pathway, and CCL5 cytokine, via TICAM1 signaling pathway, as well as IL1B secretion. [UniProt] |
| 产品亮点 | Related products: CD36 antibodies; CD36 ELISA Kits; CD36 Duos / Panels; New ELISA data calculation tool: Simplify the ELISA analysis by GainData |
| 翻译后修饰 | N-glycosylated and O-glycosylated with a ratio of 2:1. Ubiquitinated at Lys-469 and Lys-472. Ubiquitination is induced by fatty acids such as oleic acid and leads to degradation by the proteasome (PubMed:21610069, PubMed:18353783). Ubiquitination and degradation are inhibited by insulin which blocks the effect of fatty acids (PubMed:18353783). [UniProt] |
检测图片 (1) Click the Picture to Zoom In
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ARG81716 Mouse CD36 ELISA Kit standard curve image
ARG81716 Mouse CD36 ELISA Kit results of a typical standard run with optical density reading at 450 nm.
| Title | Download Link |
|---|---|
| ARG81716 Mouse CD36 ELISA Kit user's manual |
 Download
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