ARG82061
Mouse CXCL12 / SDF1 ELISA Kit
Mouse CXCL12 / SDF1 ELISA 试剂盒 for ELISA and Mouse
概述
| 产品描述 | Mouse CXCL12 / SDF1 ELISA Kit is an Enzyme Immunoassay kit for the quantification of Mouse CXCL12 / SDF1 in serum, plasma and cell culture supernatants. |
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| 反应物种 | Ms |
| 应用 | ELISA |
| 特异性 | This kit detects both SDF1 alpha and beta. |
| 靶点名称 | CXCL12 / SDF1 |
| 偶联标记 | HRP |
| 偶联标记说明 | Substrate: TMB and read at 450 nm. |
| 灵敏度 | 23.45 pg/ml |
| 样品类型 | Serum, plasma and cell culture supernatants. |
| 标准范围 | 46.9 - 3000 pg/ml |
| 样本量 | 100 µl |
| 別名 | TPAR1; SDF1; C-X-C motif chemokine 12; Pre-B cell growth-stimulating factor; TLSF; PBSF; SDF-1; Intercrine reduced in hepatomas; IRH; hSDF-1; 3-72; SCYB12; hIRH; 3-67; Stromal cell-derived factor 1 |
应用说明
| 检测时间 | 4.5 hours |
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属性
| 形式 | 96 well |
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| 存放说明 | Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | CXCL12 |
| 全名 | chemokine (C-X-C motif) ligand 12 |
| 背景介绍 | This antimicrobial gene encodes a stromal cell-derived alpha chemokine member of the intercrine family. The encoded protein functions as the ligand for the G-protein coupled receptor, chemokine (C-X-C motif) receptor 4, and plays a role in many diverse cellular functions, including embryogenesis, immune surveillance, inflammation response, tissue homeostasis, and tumor growth and metastasis. Mutations in this gene are associated with resistance to human immunodeficiency virus type 1 infections. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2014] |
| 生物功能 | Chemoattractant active on T-lymphocytes, monocytes, but not neutrophils. Activates the C-X-C chemokine receptor CXCR4 to induce a rapid and transient rise in the level of intracellular calcium ions and chemotaxis. Also binds to atypical chemokine receptor ACKR3, which activates the beta-arrestin pathway and acts as a scavenger receptor for SDF-1. SDF-1-beta(3-72) and SDF-1-alpha(3-67) show a reduced chemotactic activity. Binding to cell surface proteoglycans seems to inhibit formation of SDF-1-alpha(3-67) and thus to preserve activity on local sites. Acts as a positive regulator of monocyte migration and a negative regulator of monocyte adhesion via the LYN kinase. Stimulates migration of monocytes and T-lymphocytes through its receptors, CXCR4 and ACKR3, and decreases monocyte adherence to surfaces coated with ICAM-1, a ligand for beta-2 integrins. SDF1A/CXCR4 signaling axis inhibits beta-2 integrin LFA-1 mediated adhesion of monocytes to ICAM-1 through LYN kinase. Inhibits CXCR4-mediated infection by T-cell line-adapted HIV-1. Plays a protective role after myocardial infarction. Induces down-regulation and internalization of ACKR3 expressed in various cells. Has several critical functions during embryonic development; required for B-cell lymphopoiesis, myelopoiesis in bone marrow and heart ventricular septum formation. [UniProt] |
| 细胞定位 | Secreted. [UniProt] |
| 产品亮点 | Related products: CXCL12 antibodies; CXCL12 ELISA Kits; Related news: Detecting MMPs and their non-ECM substrates New ELISA data calculation tool: Simplify the ELISA analysis by GainData |
| 翻译后修饰 | Processed forms SDF-1-beta(3-72) and SDF-1-alpha(3-67) are produced after secretion by proteolytic cleavage of isoforms Beta and Alpha, respectively. The N-terminal processing is probably achieved by DPP4. Isoform Alpha is first cleaved at the C-terminus to yield a SDF-1-alpha(1-67) intermediate before being processed at the N-terminus. The C-terminal processing of isoform Alpha is reduced by binding to heparin and, probably, cell surface proteoglycans. [UniProt] |
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ARG82061 Mouse CXCL12 / SDF1 ELISA Kit standard curve image
ARG82061 Mouse CXCL12 / SDF1 ELISA Kit results of a typical standard run with optical density reading at 450 nm.
| Title | Download Link |
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| ARG82061 Mouse CXCL12 / SDF1 ELISA kit User manual |
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