ARG82074

Mouse MMP1 ELISA Kit

Mouse MMP1 ELISA 试剂盒 for ELISA and Mouse

概述

产品描述 ARG82074 Mouse MMP1 ELISA Kit is an Enzyme Immunoassay kit for the quantification of Mouse MMP1 in serum, plasma and cell culture supernatants.
反应物种 Ms
应用 ELISA
特异性 The following recombinant Mouse proteins were tested and exhibited no cross-reactivity or interference: Adiponectin, ApoAI, BMP1, BMP2, BMP3, BMP4, BMP5, BMP6, BMP7, CCL2, CCL4, CCL5, CRP, FGF acidic, HGF, HSP27, IGF1, IL1 alpha, IL1 beta, IL1RA, IL4, IL5, IL6, IL8, IL10, IL12, IL13, IL15, IL17C, MMP11, MMP13, IFN alpha, IFN beta, IFN gamma, MMP2, MMP3, MMP9, PDGF, PLA2G7, prolactin, TLR1, TLR2, TLR3, TLR4, TLR9, TGF beta 1, TGF beta 2, TGF beta 3, TNF alpha, TNF RI, TNF RII, VEGF, VEGF R1.
靶点名称 MMP1
偶联标记 HRP
偶联标记说明 Read at 450 nm.
灵敏度 18 pg/ml
样品类型 Serum, plasma and cell culture supernatants.
标准范围 93 - 6000 pg/ml
样本量 100 µl
精确 Intra-Assay CV: 5%; Inter-Assay CV: 10%
別名 MMP-1; CLG; Fibroblast collagenase; Matrix metalloproteinase-1; CLGN; EC 3.4.24.7; Interstitial collagenase

应用说明

检测时间 ~ 3 hours

属性

形式 96 well
存放说明 Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components.
注意事项 For laboratory research only, not for drug, diagnostic or other use.

生物信息

基因名称 MMP1
全名 matrix metallopeptidase 1
背景介绍 Proteins of the matrix metalloproteinase (MMP) family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. Most MMP's are secreted as inactive proproteins which are activated when cleaved by extracellular proteinases. This gene encodes a secreted enzyme which breaks down the interstitial collagens, types I, II, and III. The gene is part of a cluster of MMP genes which localize to chromosome 11q22.3. Alternative splicing results in multiple transcript variants.[provided by RefSeq, Mar 2009]
生物功能 Cleaves collagens of types I, II, and III at one site in the helical domain. Also cleaves collagens of types VII and X. In case of HIV infection, interacts and cleaves the secreted viral Tat protein, leading to a decrease in neuronal Tat's mediated neurotoxicity. [UniProt]
细胞定位 Secreted, extracellular space, extracellular matrix. [UniProt]
产品亮点 Related products:
MMP1 antibodies; MMP1 ELISA Kits;
Related news:
Detecting MMPs and their non-ECM substrates
New ELISA data calculation tool:
Simplify the ELISA analysis by GainData
翻译后修饰 Undergoes autolytic cleavage to two major forms (22 kDa and 27 kDa). A minor form (25 kDa) is the glycosylated form of the 22 kDa form. The 27 kDa form has no activity while the 22/25 kDa form can act as activator for collagenase.

Tyrosine phosphorylated in platelets by PKDCC/VLK. [UniProt]

检测图片 (1) Click the Picture to Zoom In

  • ARG82074 Mouse MMP1 ELISA Kit standard curve image

    ARG82074 Mouse MMP1 ELISA Kit results of a typical standard run with optical density reading at 450 nm.

Title Download Link
ARG82074 Mouse MMP1 ELISA Kit User manual download icon Download