ARG81803
Mouse MMP12 ELISA Kit
Mouse MMP12 ELISA 试剂盒 for ELISA and Mouse
参考文献1
内含物
| 货号 | 内含物名称 | 包装 | 温度 |
|---|---|---|---|
| ARG81803-001 | Antibody-coated microplate | 8 X 12 strips | 4°C. Unused strips should be sealed tightly in the air-tight pouch. |
| ARG81803-002 | Standard | 2 X 10 ng/vial | 4°C |
| ARG81803-003 | Standard/Sample diluent | 30 ml (Ready to use) | 4°C |
| ARG81803-004 | Antibody conjugate concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG81803-005 | Antibody diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG81803-006 | HRP-Streptavidin concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG81803-007 | HRP-Streptavidin diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG81803-008 | 25X Wash buffer | 20 ml | 4°C |
| ARG81803-009 | TMB substrate | 10 ml (Ready to use) | 4°C (Protect from light) |
| ARG81803-010 | STOP solution | 10 ml (Ready to use) | 4°C |
| ARG81803-011 | Plate sealer | 4 strips | Room temperature |
概述
| 产品描述 | ARG81803 Mouse MMP12 ELISA Kit is an Enzyme Immunoassay kit for the quantification of Mouse MMP12 in serum, plasma (heparin) and cell culture supernatants. |
|---|---|
| 反应物种 | Ms |
| 应用 | ELISA |
| 特异性 | There is no detectable cross-reactivity with other relevant proteins. |
| 靶点名称 | MMP12 |
| 偶联标记 | HRP |
| 偶联标记说明 | Substrate: TMB and read at 450 nm. |
| 灵敏度 | 31.25 pg/ml |
| 样品类型 | Serum, plasma (heparin) and cell culture supernatants. |
| 标准范围 | 62.5 - 4000 pg/ml |
| 样本量 | 100 µl |
| 精确 | Intra-Assay CV: 7.2%; Inter-Assay CV: 7.9% |
| 別名 | ME; EC 3.4.24.65; Macrophage metalloelastase; MMP-12; hME; MME; HME; Matrix metalloproteinase-12; Macrophage elastase |
应用说明
| 检测时间 | ~ 5 hours |
|---|
属性
| 形式 | 96 well |
|---|---|
| 存放说明 | Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | MMP12 |
| 全名 | matrix metallopeptidase 12 |
| 背景介绍 | Proteins of the matrix metalloproteinase (MMP) family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. Most MMP's are secreted as inactive proproteins which are activated when cleaved by extracellular proteinases. It is thought that the protein encoded by this gene is cleaved at both ends to yield the active enzyme, but this processing has not been fully described. The enzyme degrades soluble and insoluble elastin. It may play a role in aneurysm formation and studies in mice suggest a role in the development of emphysema. The gene is part of a cluster of MMP genes which localize to chromosome 11q22.3. [provided by RefSeq, Jul 2008] |
| 生物功能 | May be involved in tissue injury and remodeling. Has significant elastolytic activity. Can accept large and small amino acids at the P1' site, but has a preference for leucine. Aromatic or hydrophobic residues are preferred at the P1 site, with small hydrophobic residues (preferably alanine) occupying P3. [UniProt] |
| 产品亮点 | Related products: MMP12 antibodies; MMP12 ELISA Kits; Related news: Detecting MMPs and their non-ECM substrates New ELISA data calculation tool: Simplify the ELISA analysis by GainData |
检测图片 (1) Click the Picture to Zoom In
-
ARG81803 Mouse MMP12 ELISA Kit standard curve image
ARG81803 Mouse MMP12 ELISA Kit results of a typical standard run with optical density reading at 450 nm.
文献引用
MMP12 knockout prevents weight and muscle loss in tumor-bearing mice
ELISA / Mouse / Serum
| Title | Download Link |
|---|---|
| ARG81803 Mouse MMP12 ELISA Kit User's manual |
 Download
|
