ARG70316
Mouse PLGF recombinant protein (Fc-His-tagged, C-ter)
Mouse PLGF recombinant protein (Fc-His-tagged, C-ter) for Binding Activity,SDS-PAGE and Mouse
概述
| 产品描述 | HEK293 expressed, Fc-His-tagged (C-ter) Mouse PLGF recombinant protein. |
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| 反应物种 | Ms |
| 应用 | Binding, SDS-PAGE |
| 靶点名称 | PLGF |
| 物种 | Mouse |
| A.A. 序列 | Met1 - Pro158 of Mouse PLGF (NP_032853.1) with an Fc - 6X His tag at the C - terminus. |
| 表达系统 | HEK293 |
| 別名 | PGFL; PlGF; PlGF-2; SHGC-10760; Placenta growth factor; D12S1900; PLGF |
应用说明
| 应用说明 | Binding activity test: Measured by its binding ability in a functional ELISA. Immobilized Recombinant Human VEGFR1 at 500ng/ml (100 µl/well) can bind Recombinant Mouse PLGF with a linear range of 12-49 ng/ml. |
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属性
| 形式 | Powder |
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| 纯化说明 | 0.22 µm filter sterilized. Endotoxin level is <1.0 EU/µg of the protein, as determined by the LAL test. |
| 纯度 | > 87% (by SDS-PAGE) |
| 缓冲液 | PBS (pH 7.4) |
| 复溶 | Reconstitute to a concentration of 0.1 - 0.5 mg/ml in sterile distilled water. |
| 存放说明 | For long term, lyophilized protein should be stored at -20°C or -80°C. After reconstitution, aliquot and store at -20°C for up to one month, at 2-8°C for up to one week. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 基因名称 | PGF |
|---|---|
| 全名 | placental growth factor |
| 背景介绍 | This gene encodes a growth factor found in placenta which is homologous to vascular endothelial growth factor. Alternatively spliced transcripts encoding different isoforms have been found for this gene. [provided by RefSeq, Jun 2011] |
| 生物功能 | Growth factor active in angiogenesis and endothelial cell growth, stimulating their proliferation and migration. It binds to the receptor FLT1/VEGFR-1. Isoform PlGF-2 binds NRP1/neuropilin-1 and NRP2/neuropilin-2 in a heparin-dependent manner. Also promotes cell tumor growth. [UniProt] |
| 细胞定位 | Secreted. Note=The three isoforms are secreted but PlGF-2 appears to remain cell attached unless released by heparin. [UniProt] |
| 预测分子量 | 25 kDa |
| 翻译后修饰 | N-glycosylated. [UniProt] |
检测图片 (1) Click the Picture to Zoom In
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ARG70316 Mouse PLGF recombinant protein (Fc-His-tagged, C-ter) SDS-PAGE image
SDS-PAGE analysis of ARG70316 Mouse PLGF recombinant protein (Fc-His-tagged, C-ter).
