ARG81295

Mouse / Rat Insulin (Ultra sensitive) ELISA Kit

Mouse / Rat Insulin (Ultra sensitive) ELISA 试剂盒 for ELISA and Mouse,Rat

publication_link 参考文献1

概述

产品描述 ARG81295 Mouse / Rat Insulin (Ultra sensitive) ELISA Kit is an ultra sensitive Enzyme Immunoassay kit for the quantification of Mouse / Rat Insulin in serum, plasma and cell culture supernatants.
反应物种 Ms, Rat
应用 ELISA
特异性 Mouse / Rat Insulin: 100%
Hamster Insulin: Detected
Rat C-peptide, Rat pancreatic polypeptide, Porcine glucagon, Human Insulin like growth factor-I and II: Not Detected
靶点名称 Insulin
偶联标记 HRP
偶联标记说明 Substrate: TMB and read at 450 nm.
灵敏度 50 pg/ml (5 µl sample volume); 5 pg/ml (100 µl sample volume)
样品类型 Serum, plasma and cell culture supernatants.
标准范围 0.1 - 6.4 ng/ml
样本量 5 µl or 100 µl
別名 IDDM; IDDM2; IDDM1; ILPR; MODY10; Insulin; IRDN; Insulin-2; InsII; Ins-2

应用说明

检测时间 ~ 3 hours

属性

形式 96 well
存放说明 Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components.
注意事项 For laboratory research only, not for drug, diagnostic or other use.

生物信息

数据库连接

GeneID: 16334 Mouse INS

Swiss-port # P01326 Mouse Insulin

基因名称 Ins2
全名 insulin II
背景介绍 After removal of the precursor signal peptide, proinsulin is post-translationally cleaved into three peptides: the B chain and A chain peptides, which are covalently linked via two disulfide bonds to form insulin, and C-peptide. Binding of insulin to the insulin receptor (INSR) stimulates glucose uptake. A multitude of mutant alleles with phenotypic effects have been identified. There is a read-through gene, INS-IGF2, which overlaps with this gene at the 5' region and with the IGF2 gene at the 3' region. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jun 2010]
生物功能 Insulin decreases blood glucose concentration. It increases cell permeability to monosaccharides, amino acids and fatty acids. It accelerates glycolysis, the pentose phosphate cycle, and glycogen synthesis in liver. [UniProt]
产品亮点 Related products:
Insulin antibodies; Insulin ELISA Kits; Insulin Duos / Panels; Insulin recombinant proteins;
New ELISA data calculation tool:
Simplify the ELISA analysis by GainData

检测图片 (1) Click the Picture to Zoom In

  • ARG81295 Mouse Insulin (Ultra sensitive) ELISA Kit standard curve image

    ARG81295 Mouse Insulin (Ultra sensitive) ELISA Kit results of a typical standard run with optical density reading at 450 - 630 nm.

文献引用

GLP-1/GLP-1R Signaling Regulates Ovarian PCOS-Associated Granulosa Cells Proliferation and Antiapoptosis by Modification of Forkhead Box Protein O1 Phosphorylation Sites.

ELISA / Mouse / Serum

Sun Zhihua et al.
Int J Endocrinol.,  (2020)

publication_link

 

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Title Download Link
ARG81295 Mouse/Rat Insulin (Ultra sensitive) ELISA Kit User's manual download icon Download