ARG81990
Mouse TIM3 ELISA Kit
Mouse TIM3 ELISA 试剂盒 for ELISA and Mouse
内含物
| 货号 | 内含物名称 | 包装 | 温度 |
|---|---|---|---|
| ARG81990-001 | Antibody-coated microplate | 8 X 12 strips | 4°C. Unused strips should be sealed tightly in the air-tight pouch. |
| ARG81990-002 | Standard | 2 X 10 ng/vial | 4°C |
| ARG81990-003 | Standard/Sample diluent | 30 ml (Ready to use) | 4°C |
| ARG81990-004 | Antibody conjugate concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG81990-005 | Antibody diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG81990-006 | HRP-Streptavidin concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG81990-007 | HRP-Streptavidin diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG81990-008 | 25X Wash buffer | 20 ml | 4°C |
| ARG81990-009 | TMB substrate | 10 ml (Ready to use) | 4°C (Protect from light) |
| ARG81990-010 | STOP solution | 10 ml (Ready to use) | 4°C |
| ARG81990-011 | Plate sealer | 4 strips | Room temperature |
概述
| 产品描述 | ARG81990 Mouse TIM3 ELISA Kit is an Enzyme Immunoassay kit for the quantification of Mouse TIM3 in serum, plasma (heparin, EDTA) and cell culture supernatants. |
|---|---|
| 反应物种 | Ms |
| 应用 | ELISA |
| 特异性 | There is no detectable cross-reactivity with other relevant proteins. |
| 靶点名称 | TIM3 |
| 偶联标记 | HRP |
| 偶联标记说明 | Substrate: TMB and read at 450 nm. |
| 灵敏度 | 15.6 pg/ml |
| 样品类型 | Serum, plasma (heparin, EDTA) and cell culture supernatants. |
| 标准范围 | 31.2 - 2000 pg/ml |
| 样本量 | 100 µl |
| 精确 | Intra-Assay CV: 5.8%; Inter-Assay CV: 6.9% |
| 別名 | KIM-3; TIM3; TIMD-3; Tim-3; TIM-3; CD366; T-cell immunoglobulin and mucin domain-containing protein 3; TIMD3; T-cell membrane protein 3; HAVcr-2; T-cell immunoglobulin mucin receptor 3; Hepatitis A virus cellular receptor 2 |
应用说明
| 检测时间 | ~ 5 hours |
|---|
属性
| 形式 | 96 well |
|---|---|
| 存放说明 | Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 |
Swiss-port # Q8VIM0 Mouse Hepatitis A virus cellular receptor 2 homolog |
|---|---|
| 基因名称 | HAVCR2 |
| 全名 | hepatitis A virus cellular receptor 2 |
| 背景介绍 | The protein encoded by this gene belongs to the immunoglobulin superfamily, and TIM family of proteins. CD4-positive T helper lymphocytes can be divided into types 1 (Th1) and 2 (Th2) on the basis of their cytokine secretion patterns. Th1 cells are involved in cell-mediated immunity to intracellular pathogens and delayed-type hypersensitivity reactions, whereas, Th2 cells are involved in the control of extracellular helminthic infections and the promotion of atopic and allergic diseases. This protein is a Th1-specific cell surface protein that regulates macrophage activation, and inhibits Th1-mediated auto- and alloimmune responses, and promotes immunological tolerance. [provided by RefSeq, Sep 2011] |
| 生物功能 | Regulates macrophage activation. Inhibits T-helper type 1 lymphocyte (Th1)-mediated auto- and alloimmune responses and promotes immunological tolerance. May be also involved in T-cell homing. Receptor for LGALS9. [UniProt] |
| 产品亮点 | Related products: TIM3 antibodies; TIM3 ELISA Kits; New ELISA data calculation tool: Simplify the ELISA analysis by GainData |
| 翻译后修饰 | O-glycosylated with core 1 or possibly core 8 glycans. Phosphorylated on tyrosine residues; modestly increased after TCR/CD28 stimulation. Can be phosphorylated in the cytoplasmatic domain by FYN (By similarity). Phosphorylation at Tyr-265 is increased by stimulation with ligand LGALS9. [UniProt] |
检测图片 (1) Click the Picture to Zoom In
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ARG81990 Mouse TIM3 ELISA Kit standard curve image
ARG81990 Mouse TIM3 ELISA Kit results of a typical standard run with optical density reading at 450 nm.
| Title | Download Link |
|---|---|
| ARG81990 Mouse TIM3 ELISA Kit User manual |
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