ARG81674
Mouse TIMP1 ELISA Kit
Mouse TIMP1 ELISA 试剂盒 for ELISA and Mouse
内含物
| 货号 | 内含物名称 | 包装 | 温度 |
|---|---|---|---|
| ARG81674-001 | Antibody-coated microplate | 8 X 12 strips | 4°C. Unused strips should be sealed tightly in the air-tight pouch. |
| ARG81674-002 | Standard | 2 X 10 ng/vial | 4°C |
| ARG81674-003 | Standard/Sample diluent | 30 ml (Ready to use) | 4°C |
| ARG81674-004 | Antibody conjugate concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG81674-005 | Antibody diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG81674-006 | HRP-Streptavidin concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG81674-007 | HRP-Streptavidin diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG81674-008 | 25X Wash buffer | 20 ml | 4°C |
| ARG81674-009 | TMB substrate | 10 ml (Ready to use) | 4°C (Protect from light) |
| ARG81674-010 | STOP solution | 10 ml (Ready to use) | 4°C |
| ARG81674-011 | Plate sealer | 4 strips | Room temperature |
概述
| 产品描述 | ARG81674 Mouse TIMP1 ELISA Kit is an Enzyme Immunoassay kit for the quantification of Mouse TIMP1 in serum, plasma (heparin, EDTA) and cell culture supernatants. |
|---|---|
| 反应物种 | Ms |
| 应用 | ELISA |
| 特异性 | There is no detectable cross-reactivity with other relevant proteins. |
| 靶点名称 | TIMP1 |
| 偶联标记 | HRP |
| 偶联标记说明 | Substrate: TMB and read at 450 nm. |
| 灵敏度 | 15.6 pg/ml |
| 样品类型 | Serum, plasma (heparin, EDTA) and cell culture supernatants. |
| 标准范围 | 31.2 - 2000 pg/ml |
| 样本量 | 100 µl |
| 精确 | Intra-Assay CV: 5.7%; Inter-Assay CV: 7.2% |
| 別名 | Erythroid-potentiating activity; TIMP; Collagenase inhibitor; Fibroblast collagenase inhibitor; TIMP-1; EPO; CLGI; Tissue inhibitor of metalloproteinases 1; HCI; Metalloproteinase inhibitor 1; EPA |
应用说明
| 检测时间 | ~ 5 hours |
|---|
属性
| 形式 | 96 well |
|---|---|
| 存放说明 | Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | TIMP1 |
| 全名 | TIMP metallopeptidase inhibitor 1 |
| 背景介绍 | This gene belongs to the TIMP gene family. The proteins encoded by this gene family are natural inhibitors of the matrix metalloproteinases (MMPs), a group of peptidases involved in degradation of the extracellular matrix. In addition to its inhibitory role against most of the known MMPs, the encoded protein is able to promote cell proliferation in a wide range of cell types, and may also have an anti-apoptotic function. Transcription of this gene is highly inducible in response to many cytokines and hormones. In addition, the expression from some but not all inactive X chromosomes suggests that this gene inactivation is polymorphic in human females. This gene is located within intron 6 of the synapsin I gene and is transcribed in the opposite direction. [provided by RefSeq, Jul 2008] |
| 生物功能 | Metalloproteinase inhibitor that functions by forming one to one complexes with target metalloproteinases, such as collagenases, and irreversibly inactivates them by binding to their catalytic zinc cofactor. Acts on MMP1, MMP2, MMP3, MMP7, MMP8, MMP9, MMP10, MMP11, MMP12, MMP13 and MMP16. Does not act on MMP14. Also functions as a growth factor that regulates cell differentiation, migration and cell death and activates cellular signaling cascades via CD63 and ITGB1. Plays a role in integrin signaling. Mediates erythropoiesis in vitro; but, unlike IL3, it is species-specific, stimulating the growth and differentiation of only human and murine erythroid progenitors. [UniProt] |
| 产品亮点 | Related products: TIMP1 antibodies; TIMP1 ELISA Kits; Related news: Detecting MMPs and their non-ECM substrates New ELISA data calculation tool: Simplify the ELISA analysis by GainData |
| 翻译后修饰 | The activity of TIMP1 is dependent on the presence of disulfide bonds. N-glycosylated. [UniProt] |
检测图片 (1) Click the Picture to Zoom In
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ARG81674 Mouse TIMP1 ELISA Kit standard curve image
ARG81674 Mouse TIMP1 ELISA Kit results of a typical standard run with optical density reading at 450 nm.
| Title | Download Link |
|---|---|
| ARG81674 Mouse TIMP1 ELISA Kit User's manual |
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