ARG70207
Pig IL10 recombinant protein (Active) (His-tagged, C-ter)
Pig IL10 recombinant protein (Active) (His-tagged, C-ter) for SDS-PAGE
概述
| 产品描述 | E. coli expressed, His-tagged (C-ter) Active Pig IL10 recombinant protein |
|---|---|
| 应用 | SDS-PAGE |
| 靶点名称 | IL10 |
| 物种 | Pig |
| A.A. 序列 | Ser19 - Asn175 |
| 表达系统 | E. coli |
| 生物活性 | Active |
| 活性说明 | Determined by its ability to induce proliferation in MC/9‑2 cells. The ED50 for this effect is < 5 ng/mL. |
| 別名 | IL10A; GVHDS; TGIF; IL-10; Cytokine synthesis inhibitory factor; CSIF; Interleukin-10 |
属性
| 形式 | Powder |
|---|---|
| 纯化说明 | Endotoxin level is < 0.01 EU/µg of the protein, as determined by the LAL test. |
| 纯度 | > 98% (by SDS-PAGE) |
| 缓冲液 | PBS (pH 7.4) |
| 复溶 | It is recommended to reconstitute the lyophilized protein in sterile water to a concentration not less than 200 μg/mL and incubate the stock solution for at least 20 min at room temperature to make sure the protein is dissolved completely. |
| 存放说明 | For long term, lyophilized protein should be stored at -20°C or -80°C. After reconstitution, aliquot and store at -20°C or -80°C for up to one month. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 基因名称 | IL10 |
|---|---|
| 全名 | interleukin 10 |
| 背景介绍 | The protein encoded by this gene is a cytokine produced primarily by monocytes and to a lesser extent by lymphocytes. This cytokine has pleiotropic effects in immunoregulation and inflammation. It down-regulates the expression of Th1 cytokines, MHC class II Ags, and costimulatory molecules on macrophages. It also enhances B cell survival, proliferation, and antibody production. This cytokine can block NF-kappa B activity, and is involved in the regulation of the JAK-STAT signaling pathway. Knockout studies in mice suggested the function of this cytokine as an essential immunoregulator in the intestinal tract. Mutations in this gene are associated with an increased susceptibility to HIV-1 infection and rheumatoid arthritis.[provided by RefSeq, May 2011] |
| 生物功能 | Inhibits the synthesis of a number of cytokines, including IFN-gamma, IL-2, IL-3, TNF and GM-CSF produced by activated macrophages and by helper T-cells. [UniProt] |
| 细胞定位 | Secreted. [UniProt] |
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ARG70207 Pig IL10 recombinant protein (Active) (His-tagged, C-ter) SDS-PAGE image
SDS-PAGE analysis of ARG70207 Pig IL10 recombinant protein (Active) (His-tagged, C-ter).
