ARG82302

Porcine IL1 beta ELISA Kit

Porcine IL1 beta ELISA 试剂盒 for ELISA and Pig

概述

产品描述 ARG82302 Porcine IL1 beta ELISA Kit is an Enzyme Immunoassay kit for the quantification of Porcine IL1 beta in serum, plasma and cell culture supernatants.
反应物种 Pig
应用 ELISA
特异性 Not react with Porcine IL1 alpha, IL1RA and Human IL1 beta.
靶点名称 IL1 beta
偶联标记 HRP
偶联标记说明 Substrate: TMB and read at 450 nm.
灵敏度 15.6 pg/ml
样品类型 Serum, plasma and cell culture supernatants.
标准范围 31.25 - 2000 pg/ml
样本量 100 µl
精确 Intra-Assay CV: less than 9%
Inter-Assay CV: less than 9%
別名 Interleukin-1 beta; IL1-BETA; IL-1; IL-1 beta; Catabolin; IL1F2

应用说明

检测时间 ~ 3.5 hours

属性

形式 96 well
存放说明 Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components.
注意事项 For laboratory research only, not for drug, diagnostic or other use.

生物信息

基因名称 IL1B
全名 interleukin 1, beta
背景介绍 IL1 beta protein is a member of the interleukin 1 cytokine family. This cytokine is produced by activated macrophages as a proprotein, which is proteolytically processed to its active form by caspase 1 (CASP1/ICE). This cytokine is an important mediator of the inflammatory response, and is involved in a variety of cellular activities, including cell proliferation, differentiation, and apoptosis. The induction of cyclooxygenase-2 (PTGS2/COX2) by this cytokine in the central nervous system (CNS) is found to contribute to inflammatory pain hypersensitivity. This gene and eight other interleukin 1 family genes form a cytokine gene cluster on chromosome 2. [provided by RefSeq, Jul 2008]
生物功能 IL1 beta is a potent proinflammatory cytokine. Initially discovered as the major endogenous pyrogen, induces prostaglandin synthesis, neutrophil influx and activation, T-cell activation and cytokine production, B-cell activation and antibody production, and fibroblast proliferation and collagen production. Promotes Th17 differentiation of T-cells. Synergizes with IL12/interleukin-12 to induce IFNG synthesis from T-helper 1 (Th1) cells (PubMed:10653850). [UniProt]
细胞定位 Cytoplasm, cytosol. Lysosome. Secreted, exosome. Secreted. [UniProt]
产品亮点 Related products:
IL1 beta antibodies; IL1 beta ELISA Kits; IL1 beta Duos / Panels; IL1 beta recombinant proteins;
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翻译后修饰 Activation of the IL1B precursor involves a CASP1-catalyzed proteolytic cleavage. Processing and secretion are temporarily associated. [UniProt]

检测图片 (1) Click the Picture to Zoom In

  • ARG82302 Porcine IL1 beta ELISA Kit standard curve image

    ARG82302 Porcine IL1 beta ELISA Kit results of a typical standard run with optical density reading at 450 nm.

Title Download Link
ARG82302 Porcine IL1 beta ELISA kit User's manual download icon Download