ARG82111
Rat CD130 / gp130 ELISA Kit
Rat CD130 / gp130 ELISA 试剂盒 for ELISA and Rat
内含物
| 货号 | 内含物名称 | 包装 | 温度 |
|---|---|---|---|
| ARG82111-001 | Antibody-coated microplate | 8 X 12 strips | 4°C. Unused strips should be sealed tightly in the air-tight pouch. |
| ARG82111-002 | Standard | 2 X 50 ng/vial | 4°C |
| ARG82111-003 | Standard/Sample diluent | 30 ml (Ready to use) | 4°C |
| ARG82111-004 | Antibody conjugate concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG82111-005 | Antibody diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG82111-006 | HRP-Streptavidin concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG82111-007 | HRP-Streptavidin diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG82111-008 | 25X Wash buffer | 20 ml | 4°C |
| ARG82111-009 | TMB substrate | 10 ml (Ready to use) | 4°C (Protect from light) |
| ARG82111-010 | STOP solution | 10 ml (Ready to use) | 4°C |
| ARG82111-011 | Plate sealer | 4 strips | Room temperature |
概述
| 产品描述 | ARG82111 Rat CD130 / gp130 ELISA Kit is an Enzyme Immunoassay kit for the quantification of Rat CD130 / gp130 in serum, plasma (heparin, EDTA, citrate) and cell culture supernatants. |
|---|---|
| 反应物种 | Rat |
| 应用 | ELISA |
| 特异性 | There is no detectable cross-reactivity with other relevant proteins. |
| 靶点名称 | CD130 / gp130 |
| 偶联标记 | HRP |
| 偶联标记说明 | Substrate: TMB and read at 450 nm. |
| 灵敏度 | 0.39 ng/ml |
| 样品类型 | Serum, plasma (heparin, EDTA, citrate) and cell culture supernatants. |
| 标准范围 | 0.78 - 50 ng/ml |
| 样本量 | 100 µl |
| 精确 | Intra-Assay CV: 5.2%; Inter-Assay CV: 7.0% |
| 別名 | CDw130; CD130; CDW130; Interleukin-6 signal transducer; CD antigen CD130; IL-6RB; Membrane glycoprotein 130; GP130; Oncostatin-M receptor subunit alpha; IL-6R subunit beta; Interleukin-6 receptor subunit beta; gp130; IL-6 receptor subunit beta; IL-6R-beta |
应用说明
| 检测时间 | ~ 5 hours |
|---|
属性
| 形式 | 96 well |
|---|---|
| 存放说明 | Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 基因名称 | IL6ST |
|---|---|
| 全名 | interleukin 6 signal transducer |
| 背景介绍 | The protein encoded by this gene is a signal transducer shared by many cytokines, including interleukin 6 (IL6), ciliary neurotrophic factor (CNTF), leukemia inhibitory factor (LIF), and oncostatin M (OSM). This protein functions as a part of the cytokine receptor complex. The activation of this protein is dependent upon the binding of cytokines to their receptors. vIL6, a protein related to IL6 and encoded by the Kaposi sarcoma-associated herpesvirus, can bypass the interleukin 6 receptor (IL6R) and directly activate this protein. Knockout studies in mice suggest that this gene plays a critical role in regulating myocyte apoptosis. Alternatively spliced transcript variants have been described. A related pseudogene has been identified on chromosome 17. [provided by RefSeq, May 2014] |
| 生物功能 | Signal-transducing molecule. The receptor systems for IL6, LIF, OSM, CNTF, IL11, CTF1 and BSF3 can utilize gp130 for initiating signal transmission. Binds to IL6/IL6R (alpha chain) complex, resulting in the formation of high-affinity IL6 binding sites, and transduces the signal. Does not bind IL6. May have a role in embryonic development (By similarity). The type I OSM receptor is capable of transducing OSM-specific signaling events. [UniProt] |
| 细胞定位 | Isoform 1: Cell membrane; Single-pass type I membrane protein. Isoform 2: Secreted. [UniProt] |
| 产品亮点 | Related products: CD130 antibodies; CD130 ELISA Kits; New ELISA data calculation tool: Simplify the ELISA analysis by GainData |
| 翻译后修饰 | Phosphorylation of Ser-782 down-regulates cell surface expression. Heavily N-glycosylated (PubMed:11098061, PubMed:16335952, PubMed:19159218, PubMed:19139490, PubMed:11251120). Glycosylation is required for protein stability and localization in plasma membrane but not for ligand binding (PubMed:19915009). [UniProt] |
检测图片 (1) Click the Picture to Zoom In
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ARG82111 Rat CD130 / gp130 ELISA Kit standard curve image
ARG82111 Rat CD130 / gp130 ELISA Kit results of a typical standard run with optical density reading at 450 nm.
| Title | Download Link |
|---|---|
| ARG82111 Rat CD130 / gp130 ELISA Kit User's manual |
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