ARG70313
Rat IFN gamma recombinant protein (Fc-His-tagged, C-ter)
Rat IFN gamma recombinant protein (Fc-His-tagged, C-ter) for Binding Activity,SDS-PAGE and Rat
概述
| 产品描述 | HEK293 expressed, Fc-His-tagged (C-ter) Rat IFN gamma recombinant protein. |
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| 反应物种 | Rat |
| 应用 | Binding, SDS-PAGE |
| 靶点名称 | IFN gamma |
| 物种 | Rat |
| A.A. 序列 | Gln23 - Cys156 of Rat IFN gamma (NP_620235.1) with an Fc - 6X His tag at the C - terminus. |
| 表达系统 | HEK293 |
| 別名 | IFN-gamma; Interferon gamma; Immune interferon; IFG; IFI |
应用说明
| 应用说明 | Binding activity test: Measured by its binding ability in a functional ELISA. Immobilized recombinant Human IFN-gamma R1 at 3µg/ml (100µl/well) can bind recombinant Rat IFNG, the EC50 of Rat IFNG is 1 µg/ml. |
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属性
| 形式 | Powder |
|---|---|
| 纯化说明 | 0.22 µm filter sterilized. Endotoxin level is <0.1 EU/µg of the protein, as determined by the LAL test. |
| 纯度 | > 92% (by SDS-PAGE) |
| 缓冲液 | PBS (pH 7.4) |
| 复溶 | Reconstitute to a concentration of 0.1 - 0.5 mg/ml in sterile distilled water. |
| 存放说明 | For long term, lyophilized protein should be stored at -20°C or -80°C. After reconstitution, aliquot and store at -20°C for up to one month, at 2-8°C for up to one week. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 基因名称 | IFNG |
|---|---|
| 全名 | interferon, gamma |
| 背景介绍 | This gene encodes a soluble cytokine that is a member of the type II interferon class. The encoded protein is secreted by cells of both the innate and adaptive immune systems. The active protein is a homodimer that binds to the interferon gamma receptor which triggers a cellular response to viral and microbial infections. Mutations in this gene are associated with an increased susceptibility to viral, bacterial and parasitic infections and to several autoimmune diseases. [provided by RefSeq, Dec 2015] |
| 生物功能 | Produced by lymphocytes activated by specific antigens or mitogens. IFN-gamma, in addition to having antiviral activity, has important immunoregulatory functions. It is a potent activator of macrophages, it has antiproliferative effects on transformed cells and it can potentiate the antiviral and antitumor effects of the type I interferons. [UniProt] |
| 细胞定位 | Secreted. [UniProt] |
| 预测分子量 | 19 kDa |
| 翻译后修饰 | Proteolytic processing produces C-terminal heterogeneity, with proteins ending alternatively at Gly-150, Met-157 or Gly-161. [UniProt] |
检测图片 (1) Click the Picture to Zoom In
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ARG70313 Rat IFN gamma recombinant protein (Fc-His-tagged, C-ter) SDS-PAGE image
SDS-PAGE analysis of ARG70313 Rat IFN gamma recombinant protein (Fc-His-tagged, C-ter).
