ARG80235
Rat IL1 beta ELISA Kit
Rat IL1 beta ELISA 试剂盒 for ELISA and Rat
Cell Biology and Cellular Response kit; Immune System kit; Metabolism kit
内含物
| 货号 | 内含物名称 | 包装 | 温度 |
|---|---|---|---|
| ARG80235-001 | Antibody-coated microplate | 8 X 12 strips | 4°C. Unused strips should be sealed tightly in the air-tight pouch. |
| ARG80235-002 | Standard (Lyophilized) | 3 X 3.2 ng/vial | 4°C |
| ARG80235-003 | Standard diluent buffer | 20 ml | 4°C |
| ARG80235-004 | Antibody conjugate concentrate | 400 µl | 4°C |
| ARG80235-005 | Antibody diluent buffer | 16 ml | 4°C |
| ARG80235-006 | HRP-Streptavidin concentrate | 400 µl | 4°C (Protect from light) |
| ARG80235-007 | HRP-Streptavidin diluent buffer | 16 ml | 4°C |
| ARG80235-008 | 20X Wash buffer | 50 ml | 4°C |
| ARG80235-009 | TMB substrate | 12ml | 4°C (Protect from light) |
| ARG80235-010 | STOP solution | 12ml | 4°C |
| ARG80235-011 | Plate sealer | 4 strips | Room temperature |
概述
| 产品描述 | ARG80235 Rat IL1 beta ELISA Kit is an Enzyme Immunoassay kit for the quantification of Rat IL1 beta in Serum, Plasma, Cell culture supernatants. |
|---|---|
| 反应物种 | Rat |
| 应用 | ELISA |
| 特异性 | No significant cross-reactivity or interference with Human IL-2,4,1 alpha,sIL-2R,sIL-4R；mouse IL-2,4,10,1 alpha；rat GM-CSF, IL-2,4,10,1 alpha, SCF, TNF- alpha |
| 靶点名称 | IL1 beta |
| 偶联标记 | HRP |
| 偶联标记说明 | Substrate: TMB and read at 450 nm |
| 灵敏度 | 30 pg/ml |
| 样品类型 | Serum, Plasma, Cell culture supernatants |
| 标准范围 | 62.5 - 4000 pg/ml |
| 样本量 | 100 µl |
| 精确 | CV: less than10% |
| 別名 | Interleukin-1 beta; IL1-BETA; IL-1; IL-1 beta; Catabolin; IL1F2 |
应用说明
| 检测时间 | 4 hours |
|---|
属性
| 形式 | 96 well |
|---|---|
| 存放说明 | Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 基因名称 | Il1b |
|---|---|
| 全名 | interleukin 1 beta |
| 背景介绍 | IL1 beta protein is a member of the interleukin 1 cytokine family. This cytokine is produced by activated macrophages as a proprotein, which is proteolytically processed to its active form by caspase 1 (CASP1/ICE). This cytokine is an important mediator of the inflammatory response, and is involved in a variety of cellular activities, including cell proliferation, differentiation, and apoptosis. The induction of cyclooxygenase-2 (PTGS2/COX2) by this cytokine in the central nervous system (CNS) is found to contribute to inflammatory pain hypersensitivity. This gene and eight other interleukin 1 family genes form a cytokine gene cluster on chromosome 2. [provided by RefSeq, Jul 2008] |
| 生物功能 | IL1 beta is a potent proinflammatory cytokine. Initially discovered as the major endogenous pyrogen, induces prostaglandin synthesis, neutrophil influx and activation, T-cell activation and cytokine production, B-cell activation and antibody production, and fibroblast proliferation and collagen production. Promotes Th17 differentiation of T-cells. Synergizes with IL12/interleukin-12 to induce IFNG synthesis from T-helper 1 (Th1) cells (PubMed:10653850). [UniProt] |
| 产品亮点 | Related products: IL1 beta antibodies; IL1 beta ELISA Kits; IL1 beta Duos / Panels; IL1 beta recombinant proteins; Related news: HMGB1 in inflammation Inflammatory Cytokines Activation of IL-1 beta and IL-18 by Canonical Inflammasome Pathway Exploring Antiviral Immune Response RIP1 activation and pathogenesis of NASH New ELISA data calculation tool: Simplify the ELISA analysis by GainData |
| 研究领域 | Cell Biology and Cellular Response kit; Immune System kit; Metabolism kit |
| 翻译后修饰 | Activation of the IL1B precursor involves a CASP1-catalyzed proteolytic cleavage. Processing and secretion are temporarily associated. |
检测图片 (1) Click the Picture to Zoom In
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ARG80235 Rat IL1 beta ELISA Kit standard curve image
ARG80235 Rat IL1 beta ELISA Kit results of a typical standard run with optical density reading at 450 nm.
| Title | Download Link |
|---|---|
| ARG80235 Rat IL1 beta ELISA Kit User's manual |
 Download
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