ARG81796
Rat TGF beta 2 ELISA Kit
Rat TGF beta 2 ELISA 试剂盒 for ELISA and Rat
内含物
| 货号 | 内含物名称 | 包装 | 温度 |
|---|---|---|---|
| ARG81796-001 | Antibody-coated microplate | 8 X 12 strips | 4°C. Unused strips should be sealed tightly in the air-tight pouch. |
| ARG81796-002 | Standard | 2 X 10 ng/vial | 4°C |
| ARG81796-003 | Standard/Sample diluent | 30 ml (Ready to use) | 4°C |
| ARG81796-004 | Antibody conjugate concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG81796-005 | Antibody diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG81796-006 | HRP-Streptavidin concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG81796-007 | HRP-Streptavidin diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG81796-008 | 25X Wash buffer | 20 ml | 4°C |
| ARG81796-009 | TMB substrate | 10 ml (Ready to use) | 4°C (Protect from light) |
| ARG81796-010 | STOP solution | 10 ml (Ready to use) | 4°C |
| ARG81796-011 | Plate sealer | 4 strips | Room temperature |
概述
| 产品描述 | ARG81796 Rat TGF beta 2 ELISA Kit is an Enzyme Immunoassay kit for the quantification of Rat TGF beta 2 in serum, plasma (heparin, EDTA, citrate) and cell culture supernatants. |
|---|---|
| 反应物种 | Rat |
| 应用 | ELISA |
| 特异性 | There is no detectable cross-reactivity with other relevant proteins. |
| 靶点名称 | TGF beta 2 |
| 偶联标记 | HRP |
| 偶联标记说明 | Substrate: TMB and read at 450 nm. |
| 灵敏度 | 15.6 pg/ml |
| 样品类型 | Serum, plasma (heparin, EDTA, citrate) and cell culture supernatants. |
| 标准范围 | 31.2 - 2000 pg/ml |
| 样本量 | 100 µl |
| 精确 | Intra-Assay CV: 5.6%; Inter-Assay CV: 5.9% |
| 別名 | TGF-beta2; Cetermin; LDS4; Polyergin; Glioblastoma-derived T-cell suppressor factor; G-TSF; Transforming growth factor beta-2; LAP; TGF-beta-2; BSC-1 cell growth inhibitor |
应用说明
| 检测时间 | ~ 5 hours |
|---|
属性
| 形式 | 96 well |
|---|---|
| 存放说明 | Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | TGFB2 |
| 全名 | transforming growth factor, beta 2 |
| 背景介绍 | This gene encodes a member of the transforming growth factor beta (TGFB) family of cytokines, which are multifunctional peptides that regulate proliferation, differentiation, adhesion, migration, and other functions in many cell types by transducing their signal through combinations of transmembrane type I and type II receptors (TGFBR1 and TGFBR2) and their downstream effectors, the SMAD proteins. Disruption of the TGFB/SMAD pathway has been implicated in a variety of human cancers. The encoded protein is secreted and has suppressive effects of interleukin-2 dependent T-cell growth. Translocation t(1;7)(q41;p21) between this gene and HDAC9 is associated with Peters' anomaly, a congenital defect of the anterior chamber of the eye. The knockout mice lacking this gene show perinatal mortality and a wide range of developmental, including cardiac, defects. Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq, Sep 2010] |
| 生物功能 | TGF-beta 2 has suppressive effects on interleukin-2 dependent T-cell growth. [UniProt] |
| 产品亮点 | Related products: TGF beta antibodies; TGF beta ELISA Kits; TGF beta recombinant proteins; New ELISA data calculation tool: Simplify the ELISA analysis by GainData |
| 翻译后修饰 | The precursor is cleaved into mature TGF-beta-2 and LAP, which remains non-covalently linked to mature TGF-beta-2 rendering it inactive. [UniProt] |
检测图片 (1) Click the Picture to Zoom In
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ARG81796 Rat TGF beta 2 ELISA Kit standard curve image
ARG81796 Rat TGF beta 2 ELISA Kit results of a typical standard run with optical density reading at 450 nm.
| Title | Download Link |
|---|---|
| ARG81796 Rat TGF beta 2 ELISA Kit User manual |
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