ARG21616

Rat anti-Mouse IgA antibody [11-44-2]

Rat anti-Mouse IgA 抗体 [11-44-2] for ELISA,Flow cytometry,FLISA,IHC-Frozen sections,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Mouse

publication_link 参考文献1

概述

产品描述 Rat Monoclonal antibody [11-44-2] recognizes Mouse IgA
反应物种 Ms
应用 ELISA, FACS, FLISA, IHC-Fr, IHC-P, WB
宿主 Rat
克隆 Monoclonal
克隆号 11-44-2
同位型 IgG1, kappa
靶点名称 IgA
抗原物种 Mouse
偶联标记 Un-conjugated

应用说明

应用建议
应用 推荐稀释比
ELISA1 - 0.05 μg/ml
FACS0.2 - 1 μg/10^6 cells
FLISAAssay-dependent
IHC-Fr2 - 5 μg/ml
IHC-P2 - 10 μg/ml
WBAssay-dependent
应用说明 IHC-P: Antigen Retrieval: Boil tissue section in Citrate buffer (pH 6.0) for 20 min.
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

属性

形式 Liquid
缓冲液 BBS (pH 8.2)
浓度 0.5 mg/ml
存放说明 For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
注意事项 For laboratory research only, not for drug, diagnostic or other use.

检测图片 (3) Click the Picture to Zoom In

  • ARG21616 Rat anti-Mouse IgA antibody [11-44-2] IHC-P image

    Immunohistochemistry: Paraffin-embedded Mouse small intestine tissue. Antigen Retrieval: Boil tissue section in Citrate buffer (pH 6.0) for 20 min. The tissue section was stained with ARG21616 Rat anti-Mouse IgA antibody [11-44-2] (right) at 2 - 10 µg/ml dilution followed by an HRP-conjugated secondary antibody, DAB and hematoxylin. Control (left).

  • ARG21616 Rat anti-Mouse IgA antibody [11-44-2] ELISA image

    ELISA: The plate was coated with purified Mouse IgM, IgG, and IgA. Immunoglobulins were detected with serially diluted ARG21616 Rat anti-Mouse IgA antibody [11-44-2] followed by ARG21696 Mouse anti-Rat IgG1 antibody [G17E7] (HRP).

  • ARG21616 Rat anti-Mouse IgA antibody [11-44-2] IHC-Fr image

    Immunohistochemistry: Frozen section of Mouse intestine tissue stained with ARG20689 Rat IgG1 Isotype Control antibody [KLH/G1-2-2] (left) and ARG21616 Rat anti-Mouse IgA antibody [11-44-2] (right) (2-5 μg/ml) followed by ARG21695 Mouse anti-Rat IgG1 antibody [G17E7] (FITC) and DAPI.

文献引用

Weissella paramesenteroides WpK4 ameliorate the experimental amoebic colitis by increasing the expression of MUC-2 and the intestinal epithelial regeneration.

IHC-P / Mouse

Prado GKS et al.
J Appl Microbiol.,  (2020)

publication_link

 

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