ARG58795
anti-17 beta HSD 4 antibody
anti-17 beta HSD 4 antibody for IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse,Rat
概述
| 产品描述 | Rabbit Polyclonal antibody recognizes 17 beta HSD 4 |
|---|---|
| 反应物种 | Hu, Ms, Rat |
| 应用 | IHC-P, WB |
| 宿主 | Rabbit |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | 17 beta HSD 4 |
| 抗原物种 | Human |
| 抗原 | Recombinant protein corresponding to D510-L736 of Human 17 beta HSD 4. |
| 偶联标记 | Un-conjugated |
| 別名 | EC 1.1.1.n12; Multifunctional protein 2; Peroxisomal multifunctional enzyme type 2; SDR8C1; EC 4.2.1.107; 17-beta-HSD 4; 3R; MFE-2; PRLTS1; 3-alpha,7-alpha,12-alpha-trihydroxy-5-beta-cholest-24-enoyl-CoA hydratase; Short chain dehydrogenase/reductase family 8C member 1; 17-beta-hydroxysteroid dehydrogenase 4; DBP; MPF-2; EC 4.2.1.119; D-bifunctional protein |
应用说明
| 应用建议 |
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| 应用说明 | IHC-P: Antigen Retrieval: Heat mediated was performed in Citrate buffer (pH 6.0) for 20 min. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Affinity purification with immunogen. |
| 缓冲液 | 0.9% NaCl, 0.2% Na2HPO4, 0.05% Sodium azide and 5% BSA. |
| 抗菌剂 | 0.05% Sodium azide |
| 稳定剂 | 5% BSA |
| 浓度 | 0.5 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | HSD17B4 |
| 全名 | hydroxysteroid (17-beta) dehydrogenase 4 |
| 背景介绍 | The protein encoded by this gene is a bifunctional enzyme that is involved in the peroxisomal beta-oxidation pathway for fatty acids. It also acts as a catalyst for the formation of 3-ketoacyl-CoA intermediates from both straight-chain and 2-methyl-branched-chain fatty acids. Defects in this gene that affect the peroxisomal fatty acid beta-oxidation activity are a cause of D-bifunctional protein deficiency (DBPD). An apparent pseudogene of this gene is present on chromosome 8. Multiple alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. [provided by RefSeq, May 2014] |
| 生物功能 | Bifunctional enzyme acting on the peroxisomal beta-oxidation pathway for fatty acids. Catalyzes the formation of 3-ketoacyl-CoA intermediates from both straight-chain and 2-methyl-branched-chain fatty acids. [UniProt] |
| 细胞定位 | Peroxisome. [UniProt] |
| 预测分子量 | 80 kDa |
检测图片 (4) Click the Picture to Zoom In
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ARG58795 anti-17 beta HSD 4 antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Human intestinal cancer tissues. Antigen Retrieval: Heat mediated was performed in Citrate buffer (pH 6.0, epitope retrieval solution) for 20 min. The tissue section was blocked with 10% goat serum. The tissue section was then stained with ARG58795 anti-17 beta HSD 4 antibody at 1 µg/ml, overnight at 4°C.
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ARG58795 anti-17 beta HSD 4 antibody WB image
Western blot: 50 µg of samples under reducing conditions. Rat liver, Mouse liver, Mouse heart, Mouse testis, MCF-7 and HeLa lysates stained with ARG58795 anti-17 beta HSD 4 antibody at 0.5 µg/ml, overnight at 4°C.
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ARG58795 anti-17 beta HSD 4 antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Human lung cancer tissues. Antigen Retrieval: Heat mediated was performed in Citrate buffer (pH 6.0, epitope retrieval solution) for 20 min. The tissue section was blocked with 10% goat serum. The tissue section was then stained with ARG58795 anti-17 beta HSD 4 antibody at 1 µg/ml, overnight at 4°C.
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ARG58795 anti-17 beta HSD 4 antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Human mammary cancer tissues. Antigen Retrieval: Heat mediated was performed in Citrate buffer (pH 6.0, epitope retrieval solution) for 20 min. The tissue section was blocked with 10% goat serum. The tissue section was then stained with ARG58795 anti-17 beta HSD 4 antibody at 1 µg/ml, overnight at 4°C.
