ARG59384
anti-AGO2 / Argonaute 2 antibody
anti-AGO2 / Argonaute 2 antibody for IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse,Rat
概述
| 产品描述 | Rabbit Polyclonal antibody recognizes AGO2 / Argonaute 2 |
|---|---|
| 反应物种 | Hu, Ms, Rat |
| 应用 | IHC-P, WB |
| 宿主 | Rabbit |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | AGO2 / Argonaute 2 |
| 抗原物种 | Human |
| 抗原 | Synthetic peptide within aa. 1-100 of Human AGO2 / Argonaute 2 (NP_036286.2). |
| 偶联标记 | Un-conjugated |
| 別名 | EC 3.1.26.n2; eIF2C 2; PPD; Protein slicer; Argonaute RISC catalytic component 2; EIF2C2; Argonaute2; PAZ Piwi domain protein; Q10; hAgo2; Protein argonaute-2; Eukaryotic translation initiation factor 2C 2; eIF-2C 2 |
应用说明
| 应用建议 |
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| 应用说明 | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||
| 阳性对照 | Mouse heart | ||||||
| 实际分子量 | 100 kDa |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Affinity purified. |
| 缓冲液 | PBS (pH 7.3), 0.02% Sodium azide and 50% Glycerol. |
| 抗菌剂 | 0.02% Sodium azide |
| 稳定剂 | 50% Glycerol |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | AGO2 |
| 全名 | argonaute RISC catalytic component 2 |
| 背景介绍 | This gene encodes a member of the Argonaute family of proteins which play a role in RNA interference. The encoded protein is highly basic, and contains a PAZ domain and a PIWI domain. It may interact with dicer1 and play a role in short-interfering-RNA-mediated gene silencing. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2009] |
| 生物功能 | Required for RNA-mediated gene silencing (RNAi) by the RNA-induced silencing complex (RISC). The 'minimal RISC' appears to include AGO2 bound to a short guide RNA such as a microRNA (miRNA) or short interfering RNA (siRNA). These guide RNAs direct RISC to complementary mRNAs that are targets for RISC-mediated gene silencing. The precise mechanism of gene silencing depends on the degree of complementarity between the miRNA or siRNA and its target. Binding of RISC to a perfectly complementary mRNA generally results in silencing due to endonucleolytic cleavage of the mRNA specifically by AGO2. Binding of RISC to a partially complementary mRNA results in silencing through inhibition of translation, and this is independent of endonuclease activity. May inhibit translation initiation by binding to the 7-methylguanosine cap, thereby preventing the recruitment of the translation initiation factor eIF4-E. May also inhibit translation initiation via interaction with EIF6, which itself binds to the 60S ribosomal subunit and prevents its association with the 40S ribosomal subunit. The inhibition of translational initiation leads to the accumulation of the affected mRNA in cytoplasmic processing bodies (P-bodies), where mRNA degradation may subsequently occur. In some cases RISC-mediated translational repression is also observed for miRNAs that perfectly match the 3' untranslated region (3'-UTR). Can also up-regulate the translation of specific mRNAs under certain growth conditions. Binds to the AU element of the 3'-UTR of the TNF (TNF-alpha) mRNA and up-regulates translation under conditions of serum starvation. Also required for transcriptional gene silencing (TGS), in which short RNAs known as antigene RNAs or agRNAs direct the transcriptional repression of complementary promoter regions. [UniProt] |
| 细胞定位 | Cytoplasm, P-body. Nucleus. Note=Translational repression of mRNAs results in their recruitment to P-bodies. Translocation to the nucleus requires IMP8. [UniProt] |
| 预测分子量 | 97 kDa |
| 翻译后修饰 | Hydroxylated. 4-hydroxylation appears to enhance protein stability but is not required for miRNA-binding or endonuclease activity. [UniProt] |
检测图片 (2) Click the Picture to Zoom In
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ARG59384 anti-AGO2 / Argonaute 2 antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Mouse liver stained with ARG59384 anti-AGO2 / Argonaute 2 antibody at 1:100 dilution.
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ARG59384 anti-AGO2 / Argonaute 2 antibody WB image
Western blot: 25 µg of Mouse heart lysate stained with ARG59384 anti-AGO2 / Argonaute 2 antibody at 1:1000 dilution.
