ARG63951
anti-AIF1 / Iba 1 antibody
anti-AIF1 / Iba 1 antibody for Flow cytometry,ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse
Cell Biology and Cellular Response antibody; Immune System antibody; Metabolism antibody; Neuroscience antibody; Activated Macrophage/Microglia Study antibody; Neuroinflammation Study antibody; Macroglial Marker antibody
概述
| 产品描述 | Goat Polyclonal antibody recognizes AIF1 / Iba 1 |
|---|---|
| 反应物种 | Hu, Ms |
| 预测物种 | Dog |
| 应用 | FACS, ICC/IF, IHC-P, WB |
| 特异性 | This antibody is expected to recognise isoform 3 (NP_001614.3) only. |
| 宿主 | Goat |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | AIF1 / Iba 1 |
| 抗原物种 | Human |
| 抗原 | C-NKQFLDDPKYSSDED |
| 偶联标记 | Un-conjugated |
| 別名 | Ionized calcium-binding adapter molecule 1; Allograft inflammatory factor 1; IBA1; IRT-1; IRT1; AIF-1; Protein G1 |
应用说明
| 应用建议 |
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| 应用说明 | IHC-P: Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0). WB: Recommend incubate at RT for 1h. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Purified from goat serum by antigen affinity chromatography. |
| 缓冲液 | Tris saline (pH 7.3), 0.02% Sodium azide and 0.5% BSA. |
| 抗菌剂 | 0.02% Sodium azide |
| 稳定剂 | 0.5% BSA |
| 浓度 | 0.5 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 背景介绍 | AIF1 / Iba 1 is a protein that binds actin and calcium. This gene is induced by cytokines and interferon and may promote macrophage activation and growth of vascular smooth muscle cells and T-lymphocytes. Polymorphisms in this gene may be associated with systemic sclerosis. Alternative splicing results in multiple transcript variants, but the full-length and coding nature of some of these variants is not certain. [provided by RefSeq, Jan 2016] |
| 生物功能 | AIF1 / Iba 1 is an Actin-binding protein. It enhances membrane ruffling and RAC activation. Enhances the actin-bundling activity of LCP1. Binds calcium. Plays a role in RAC signaling and in phagocytosis. May play a role in macrophage activation and function. Promotes the proliferation of vascular smooth muscle cells and of T-lymphocytes. Enhances lymphocyte migration. Plays a role in vascular inflammation. [UniProt] |
| 研究领域 | Cell Biology and Cellular Response antibody; Immune System antibody; Metabolism antibody; Neuroscience antibody; Activated Macrophage/Microglia Study antibody; Neuroinflammation Study antibody; Macroglial Marker antibody |
| 预测分子量 | 17 kDa |
| 翻译后修饰 | Phosphorylated on serine residues. |
检测图片 (4) Click the Picture to Zoom In
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ARG63951 anti-AIF1 / Iba 1 antibody ICC/IF image
Immunofluorescence: Paraformaldehyde fixed Caco-2 cells permeabilized with 0.15% Triton. Cells were stained with ARG63951 anti-AIF1 / Iba 1 antibody (green) at 10 µg/ml dilution for 1 hour. DAPI (blue) for nuclear staining. Negative control: Unimmunized goat IgG (green) at 10 µg/ml dilution.
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ARG63951 anti-AIF1 / Iba 1 antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Mouse brain tissue. Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0). The tissue section was stained with ARG63951 anti-AIF1 / Iba 1 antibody at 2 µg/ml dilution, followed by HRP-staining.
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ARG63951 anti-AIF1 / Iba 1 antibody WB image
Western blot: 35 µg of Human frontal cortex lysate (in RIPA buffer) stained with ARG63951 anti-AIF1 / Iba 1 antibody at 0.5 µg/ml dilution and incubated at RT for 1 hour.
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ARG63951 anti-AIF1 / Iba 1 antibody FACS image
Flow Cytometry: Paraformaldehyde-fixed K562 cells permeabilized with 0.5% Triton. Cells were stained with ARG63951 anti-AIF1 / Iba 1 antibody (blue line) at 10 µg/ml dilution for 1 hour, followed by incubation with Alexa FluorR 488 labelled secondary antibody. IgG control: Unimmunized goat IgG (black line).
