ARG66956
anti-AIF1 / Iba 1 antibody
anti-AIF1 / Iba 1 antibody for IHC-Frozen sections,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse,Rat
Cell Biology and Cellular Response antibody; Immune System antibody; Metabolism antibody; Neuroscience antibody; Activated Macrophage/Microglia Study antibody; Neuroinflammation Study antibody; Macroglial Marker antibody
概述
| 产品描述 | Rabbit Polyclonal antibody recognizes AIF1 / Iba 1 |
|---|---|
| 反应物种 | Hu, Ms, Rat |
| 应用 | IHC-Fr, IHC-P, WB |
| 宿主 | Rabbit |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | AIF1 / Iba 1 |
| 抗原物种 | Human |
| 抗原 | Synthetic peptide around the C-terminal region of Human AIF1 / Iba1. |
| 偶联标记 | Un-conjugated |
| 別名 | Ionized calcium-binding adapter molecule 1; Allograft inflammatory factor 1; IBA1; IRT-1; IRT1; AIF-1; Protein G1 |
应用说明
| 应用建议 |
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| 应用说明 | IHC-P: Antigen Retrieval: Boil tissue section in Sodium Citrate buffer (pH 6.0) followed by cooling at RT for 20 min. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
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| 阳性对照 | Brain tissue | ||||||||
| 实际分子量 | ~15 kDa |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Affinity purified. |
| 缓冲液 | 100 mM Tris Glycine (pH 7.0), 0.025% ProClin 300, 20% Glycerol and 1% BSA. |
| 抗菌剂 | 0.025% ProClin 300 |
| 稳定剂 | 20% Glycerol and 1% BSA |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | AIF1 |
| 全名 | allograft inflammatory factor 1 |
| 背景介绍 | AIF1 / Iba 1 is a protein that binds actin and calcium. This gene is induced by cytokines and interferon and may promote macrophage activation and growth of vascular smooth muscle cells and T-lymphocytes. Polymorphisms in this gene may be associated with systemic sclerosis. Alternative splicing results in multiple transcript variants, but the full-length and coding nature of some of these variants is not certain. [provided by RefSeq, Jan 2016] |
| 生物功能 | AIF1 / Iba 1 is an Actin-binding protein. It enhances membrane ruffling and RAC activation. Enhances the actin-bundling activity of LCP1. Binds calcium. Plays a role in RAC signaling and in phagocytosis. May play a role in macrophage activation and function. Promotes the proliferation of vascular smooth muscle cells and of T-lymphocytes. Enhances lymphocyte migration. Plays a role in vascular inflammation. [UniProt] |
| 产品亮点 | Related Antibody Duos and Panels: ARG30324 Neuroinflammation Antibody Panel Related products: AIF1 antibodies; AIF1 Duos / Panels; Anti-Rabbit IgG secondary antibodies; |
| 研究领域 | Cell Biology and Cellular Response antibody; Immune System antibody; Metabolism antibody; Neuroscience antibody; Activated Macrophage/Microglia Study antibody; Neuroinflammation Study antibody; Macroglial Marker antibody |
| 预测分子量 | 17 kDa |
| 翻译后修饰 | Phosphorylated on serine residues. [UniProt] |
检测图片 (5) Click the Picture to Zoom In
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ARG66956 anti-AIF1 / Iba 1 antibody IHC-P image
Immunohistochemistry: Paraffin-embedded human lymoph node carcinoma tissue stained with ARG66956 anti-AIF1 / Iba 1 antibody at 1:200 dilution.
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ARG66956 anti-AIF1 / Iba 1 antibody IHC-P image
Immunohistochemistry: Paraffin-embedded mouse brain tissue stained with ARG66956 anti-AIF1 / Iba 1 antibody at 1:200 dilution. Antigen Retrieval: Boil tissue section in Citrate buffer (pH 6.0).
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ARG66956 anti-AIF1 / Iba 1 antibody WB image
Western blot: 45 µg of THP-1 cell lysate stained with ARG66956 anti-AIF1 / Iba 1 antibody at 1:500 dilution, overnight at 4°C.
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ARG66956 anti-AIF1 / Iba 1 antibody IHC-Fr image
Immunohistochemistry: Frozen section of Mouse hippocampus tissue stained with ARG66956 anti-AIF1 / Iba 1 antibody (green) at 1:200 dilution. Nuclei are stained with DAPI (blue).
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ARG66956 anti-AIF1 / Iba 1 antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Rat brain tissue stained with ARG66956 anti-AIF1 / Iba 1 antibody at 1:200 dilution.
