ARG63254
anti-ARIH1 / HHARI antibody
anti-ARIH1 / HHARI antibody for IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human
Cell Biology and Cellular Response antibody; Metabolism antibody; Signaling Transduction antibody
概述
| 产品描述 | Goat Polyclonal antibody recognizes ARIH1 / HHARI |
|---|---|
| 反应物种 | Hu |
| 预测物种 | Ms, Cow |
| 应用 | IHC-P, WB |
| 宿主 | Goat |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | ARIH1 / HHARI |
| 抗原物种 | Human |
| 抗原 | C-HEGYEKDLWEYIED |
| 偶联标记 | Un-conjugated |
| 別名 | UbcH7-binding protein; HARI; EC 6.3.2.-; E3 ubiquitin-protein ligase ARIH1; ARI-1; Ubiquitin-conjugating enzyme E2-binding protein 1; Protein ariadne-1 homolog; MOP-6; H7-AP2; UbcM4-interacting protein; HHARI; ARI; Monocyte protein 6; UBCH7BP |
应用说明
| 应用建议 |
|
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|---|---|---|---|---|---|---|---|
| 应用说明 | WB: Recommend incubate at RT for 1h. IHC-P: Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0). * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Purified from goat serum by antigen affinity chromatography. |
| 缓冲液 | Tris saline (pH 7.3), 0.02% Sodium azide and 0.5% BSA. |
| 抗菌剂 | 0.02% Sodium azide |
| 稳定剂 | 0.5% BSA |
| 浓度 | 0.5 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | ARIH1 |
| 全名 | ariadne RBR E3 ubiquitin protein ligase 1 |
| 生物功能 | E3 ubiquitin-protein ligase, which catalyzes polyubiquitination of target proteins together with ubiquitin-conjugating enzyme E2 UBE2L3. May play a role in protein translation by mediating polyubiquitination of EIF4E2, leading to its subsequent degradation. Acts as the ligase involved in ISGylation of EIF4E2. [UniProt] |
| 研究领域 | Cell Biology and Cellular Response antibody; Metabolism antibody; Signaling Transduction antibody |
| 预测分子量 | 64 kDa |
检测图片 (2) Click the Picture to Zoom In
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ARG63254 anti-ARIH1 / HHARI antibody WB image
Western Blot: U937 lysate (RIPA buffer, 30µg total protein per lane) stained with ARG63254 anti-ARIH1 / HHARI antibody at 0.5 µg/ml dilution.
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ARG63254 anti-ARIH1 / HHARI antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Human adrenal gland tissue. Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0). The tissue section was stained with ARG63254 anti-ARIH1 / HHARI antibody at 5 µg/ml dilution followed by AP-staining.
