ARG54919
anti-ATG101 antibody
anti-ATG101 antibody for ELISA,ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse,Rat
Cell Biology and Cellular Response antibody; Cell Death antibody
概述
| 产品描述 | Rabbit Polyclonal antibody recognizes ATG101 |
|---|---|
| 反应物种 | Hu, Ms, Rat |
| 应用 | ELISA, ICC/IF, IHC-P, WB |
| 宿主 | Rabbit |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | ATG101 |
| 抗原物种 | Human |
| 抗原 | Synthetic peptide (16 aa) within aa. 80-130 of Human ATG101. |
| 偶联标记 | Un-conjugated |
| 別名 | Autophagy-related protein 101; C12orf44 |
应用说明
| 应用建议 |
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| 应用说明 | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||||||
| 阳性对照 | Human Liver Tissue Lysate |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Affinity purification with immunogen. |
| 缓冲液 | PBS and 0.02% Sodium azide |
| 抗菌剂 | 0.02% Sodium azide |
| 浓度 | 1 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | ATG101 |
| 全名 | autophagy related 101 |
| 背景介绍 | ATG101 Antibody: Autophagy, the process of bulk degradation of cellular proteins through an autophagosomic-lysosomal pathway is important for normal growth control and may be defective in tumor cells. It is involved in the preservation of cellular nutrients under starvation conditions as well as the normal turnover of cytosolic components. This process is negatively regulated by TOR (Target of rapamycin) through phosphorylation of autophagy protein ATG1. ATG101 is a recently discovered protein that stabilizes ATG13, another autophagy protein that forms a complex with the mammalian homologs of ATG1, ULK1 and ULK2, and with FIP200. This complex is a target of TOR phosphorylation under normal conditions; inhibition of TOR by rapamycin or leucine deprivation leads to dephosphorylation of ATG13, ULK1 and ULK2, which then leads to autophagy. ATG101 also interacts with ULK1 and is essential for autophagy. |
| 生物功能 | Autophagy factor required for autophagosome formation. Stabilizes ATG13, protecting it from proteasomal degradation. [UniProt] |
| 研究领域 | Cell Biology and Cellular Response antibody; Cell Death antibody |
| 预测分子量 | 25 kDa |
检测图片 (3) Click the Picture to Zoom In
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ARG54919 anti-ATG101 antibody WB image
Western blot: human liver tissue lysate stained with ARG54919 anti-ATG101 antibody at (A) 1 and (B) 2 ug/ml dilution.
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ARG54919 anti-ATG101 antibody IHC image
Immunohistochemistry: ATG101 in mouse liver tissue stained with ARG54919 anti-ATG101 antibody at 20 ug/ml dilution.
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ARG54919 anti-ATG101 antibody IHC image
Immunohistochemistry: ATG101 in mouse liver stained with ARG54919 anti-ATG101 antibody at 5 ug/ml dilution.
