ARG42494
anti-ATG12 antibody
anti-ATG12 antibody for ICC/IF,IHC-Formalin fixed sections,IHC-Frozen sections,Western blot and Dog,Human,Monkey,Mouse,Rat
概述
| 产品描述 | Goat Polyclonal antibody recognizes ATG12 |
|---|---|
| 反应物种 | Hu, Ms, Rat, Dog, Mk |
| 应用 | ICC/IF, IHC-F, IHC-Fr, WB |
| 宿主 | Goat |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | ATG12 |
| 抗原物种 | Human |
| 抗原 | Purified recombinant peptide within aa.65 to the N-terminus of Human ATG12. |
| 偶联标记 | Un-conjugated |
| 別名 | Ubiquitin-like protein ATG12; FBR93; HAPG12; APG12-like; Autophagy-related protein 12; APG12L; APG12 |
应用说明
| 应用建议 |
|
||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| 应用说明 | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Affinity purification with immunogen. |
| 缓冲液 | PBS, 0.05% Sodium azide and 20% Glycerol. |
| 抗菌剂 | 0.05% Sodium azide |
| 稳定剂 | 20% Glycerol |
| 浓度 | 3 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | ATG12 |
| 全名 | autophagy related 12 |
| 背景介绍 | Autophagy is a process of bulk protein degradation in which cytoplasmic components, including organelles, are enclosed in double-membrane structures called autophagosomes and delivered to lysosomes or vacuoles for degradation. ATG12 is the human homolog of a yeast protein involved in autophagy (Mizushima et al., 1998 [PubMed 9852036]).[supplied by OMIM, Mar 2008] |
| 生物功能 | Ubiquitin-like protein involved in autophagy vesicles formation. Conjugation with ATG5 through a ubiquitin-like conjugating system involving also ATG7 as an E1-like activating enzyme and ATG10 as an E2-like conjugating enzyme, is essential for its function. The ATG12-ATG5 conjugate acts as an E3-like enzyme which is required for lipidation of ATG8 family proteins and their association to the vesicle membranes. (Microbial infection) May act as a proviral factor. In association with ATG5, negatively regulates the innate antiviral immune response by impairing the type I IFN production pathway upon vesicular stomatitis virus (VSV) infection (PubMed:17709747). Required for the translation of incoming hepatitis C virus (HCV) RNA and, thereby, for the initiation of HCV replication, but not required once infection is established (PubMed:19666601). [UniProt] |
| 细胞定位 | Cytoplasm. Preautophagosomal structure membrane; Peripheral membrane protein. Note=TECPR1 recruits the ATG12-ATG5 conjugate to the autolysosomal membrane. [UniProt] |
| 预测分子量 | 15 kDa |
| 翻译后修饰 | Acetylated by EP300. [UniProt] |
检测图片 (4) Click the Picture to Zoom In
-
ARG42494 anti-ATG12 antibody IHC-P image
Immunohistochemistry: Human pancreas stained with ARG42494 anti-ATG12 antibody at 1:500 dilution.
-
ARG42494 anti-ATG12 antibody ICC/IF image
Immunofluorescence: Hepa1-6 cells were fixed with methanol. Cells were stained with ARG42494 anti-ATG12 antibody (green) at 1:50 dilution. Nuclear staining (blue).
-
ARG42494 anti-ATG12 antibody WB image
Western blot: GFP-ATG12 transfected HEK293 cells. 100 µg of cell lysate stained with ARG42494 anti-ATG12 antibody at 1:500 dilution.
-
ARG42494 anti-ATG12 antibody IHC-P image
Immunohistochemistry: Mouse kidney stained with ARG42494 anti-ATG12 antibody at 1:500 dilution.
