ARG55112
anti-ATG13 antibody
anti-ATG13 antibody for ELISA,ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse,Rat
Cancer antibody; Cell Death antibody; Metabolism antibody
概述
| 产品描述 | Rabbit Polyclonal antibody recognizes ATG13 |
|---|---|
| 反应物种 | Hu, Ms, Rat |
| 应用 | ELISA, ICC/IF, IHC-P, WB |
| 特异性 | Multiple isoforms of ATG13 are known to exist. |
| 宿主 | Rabbit |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | ATG13 |
| 抗原物种 | Human |
| 抗原 | Synthetic peptide (15 aa) within aa. 450-500 of Human ATG13 protein. |
| 偶联标记 | Un-conjugated |
| 別名 | KIAA0652; Autophagy-related protein 13; PARATARG8 |
应用说明
| 应用建议 |
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| 应用说明 | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||||||
| 阳性对照 | Rat Heart Tissue Lysate | ||||||||||
| 实际分子量 | ~ 49 kDa |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Affinity purification with immunogen. |
| 缓冲液 | PBS and 0.02% Sodium azide |
| 抗菌剂 | 0.02% Sodium azide |
| 浓度 | 1 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | ATG13 |
| 全名 | autophagy related 13 |
| 背景介绍 | ATG13 Antibody: Autophagy, the process of bulk degradation of cellular proteins through an autophagosomic-lysosomal pathway is important for normal growth control and may be defective in tumor cells. It is involved in the preservation of cellular nutrients under starvation conditions as well as the normal turnover of cytosolic components. This process is negatively regulated by TOR (Target of rapamycin) through phosphorylation of autophagy protein ATG1. ATG13 forms a complex with ULK1 and ULK2, the mammalian homologs of ATG1, and with FIP200. This complex is a target of TOR phosphorylation under normal conditions; inhibition of TOR by rapamycin or leucine deprivation leads to dephosphorylation of ATG13, ULK1 and ULK2, which then leads to autophagy. Knockdown of ATG13 inhibits autophagosome formation. |
| 生物功能 | Autophagy factor required for autophagosome formation and mitophagy. Target of the TOR kinase signaling pathway that regulates autophagy through the control of the phosphorylation status of ATG13 and ULK1, and the regulation of the ATG13-ULK1-RB1CC1 complex. Through its regulation of ULK1 activity, plays a role in the regulation of the kinase activity of mTORC1 and cell proliferation. [UniProt] |
| 研究领域 | Cancer antibody; Cell Death antibody; Metabolism antibody |
| 预测分子量 | 57 kDa |
| 翻译后修饰 | Phosphorylated by ULK1, ULK2 and mTOR. Phosphorylation status depends on nutrient-rich conditions; dephosphorylated during starvation or following treatment with rapamycin. ULK1-mediated phosphorylation of ATG13 at Ser-355 is required for efficient clearance of depolarized mitochondria. |
检测图片 (3) Click the Picture to Zoom In
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ARG55112 anti-ATG13 antibody WB image
Western blot: rat heart tissue lysate stained with ARG55112 anti-ATG13 antibody at 1 ug/ml dilution in (A) the absence and (B) the presence of blocking peptide.
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ARG55112 anti-ATG13 antibody IHC image
Immunohistochemistry: ATG13 in Mouse Heart tissue stained with ARG55112 anti-ATG13 antibody at 20 ug/ml dilution.
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ARG55112 anti-ATG13 antibody IHC image
Immunohistochemistry: ATG13 in mouse heart stained with ARG55112 anti-ATG13 antibody at 5 ug/ml dilution.
