ARG57291
anti-ATG13 antibody
anti-ATG13 antibody for ICC/IF,Western blot and Human,Mouse,Rat
1
概述
| 产品描述 | Rabbit Polyclonal antibody recognizes ATG13 |
|---|---|
| 反应物种 | Hu, Ms, Rat |
| 应用 | ICC/IF, WB |
| 宿主 | Rabbit |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | ATG13 |
| 抗原物种 | Human |
| 抗原 | Recombinant Protein of Human ATG13. |
| 偶联标记 | Un-conjugated |
| 別名 | KIAA0652; Autophagy-related protein 13; PARATARG8 |
应用说明
| 应用建议 |
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| 应用说明 | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Affinity purification with immunogen. |
| 缓冲液 | PBS (pH 7.3), 0.02% Sodium azide and 50% Glycerol. |
| 抗菌剂 | 0.02% Sodium azide |
| 稳定剂 | 50% Glycerol |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | ATG13 |
| 全名 | autophagy related 13 |
| 生物功能 | Autophagy factor required for autophagosome formation and mitophagy. Target of the TOR kinase signaling pathway that regulates autophagy through the control of the phosphorylation status of ATG13 and ULK1, and the regulation of the ATG13-ULK1-RB1CC1 complex. Through its regulation of ULK1 activity, plays a role in the regulation of the kinase activity of mTORC1 and cell proliferation. [UniProt] |
| 预测分子量 | 57 kDa |
| 翻译后修饰 | Phosphorylated by ULK1, ULK2 and mTOR. Phosphorylation status depends on nutrient-rich conditions; dephosphorylated during starvation or following treatment with rapamycin. ULK1-mediated phosphorylation of ATG13 at Ser-355 is required for efficient clearance of depolarized mitochondria. |
检测图片 (2) Click the Picture to Zoom In
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ARG57291 anti-ATG13 antibody WB image
Western blot: 20 µg of HeLa and 3T3 cell lysates stained with ARG57291 anti-ATG13 antibody at 1:1000 dilution.
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ARG57291 anti-ATG13 antibody ICC/IF image
Immunofluorescence: A549 cells stained with ARG57291 anti-ATG13 antibody.
客户反馈
Excellent
anti-ATG13 antibody
Application：WB
Sample：HeLa and 3T3
Sample Loading Amount：20 µg
Primary Antibody Dilution Factor：1:1000
Primary Antibody Incubation Time：overnight
Primary Antibody Incubation Temperature：4 ºC
