ARG54957
anti-ATG16L antibody
anti-ATG16L antibody for ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse
Cancer antibody; Cell Biology and Cellular Response antibody; Cell Death antibody; Metabolism antibody; Neuroscience antibody
概述
| 产品描述 | Rabbit Polyclonal antibody recognizes ATG16L |
|---|---|
| 反应物种 | Hu, Ms |
| 应用 | ICC/IF, IHC-P, WB |
| 宿主 | Rabbit |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | ATG16L |
| 抗原物种 | Human |
| 抗原 | KLH-conjugated synthetic peptide corresponding to aa. 161-190 of Human ATG16L. |
| 偶联标记 | Un-conjugated |
| 別名 | IBD10; APG16L; ATG16L; WDR30; Autophagy-related protein 16-1; APG16-like 1; ATG16A |
应用说明
| 应用建议 |
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| 应用说明 | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||||
| 阳性对照 | NCI-H460 |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS. |
| 缓冲液 | PBS and 0.09% (W/V) Sodium azide |
| 抗菌剂 | 0.09% (W/V) Sodium azide |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | ATG16L1 |
| 全名 | autophagy related 16-like 1 |
| 背景介绍 | The protein encoded by this gene is part of a large protein complex that is necessary for autophagy, the major process by which intracellular components are targeted to lysosomes for degradation. Defects in this gene are a cause of susceptibility to inflammatory bowel disease type 10 (IBD10). Several transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Jun 2010] |
| 生物功能 | Plays an essential role in autophagy: interacts with ATG12-ATG5 to mediate the conjugation of phosphatidylethanolamine (PE) to LC3 (MAP1LC3A, MAP1LC3B or MAP1LC3C), to produce a membrane-bound activated form of LC3 named LC3-II. Thereby, controls the elongation of the nascent autophagosomal membrane. [UniProt] |
| 细胞定位 | Cytoplasm. Preautophagosomal structure membrane; Peripheral membrane protein. Note=Recruited to omegasomes membranes by WIPI2 Omegasomes are endoplasmic reticulum connected strutures at the origin of preautophagosomal structures. Localized to preautophagosomal structure (PAS) where it is involved in the membrane targeting of ATG5. Localizes also to discrete punctae along the ciliary axoneme. {ECO:0000250|UniProtKB:Q8C0J2} |
| 研究领域 | Cancer antibody; Cell Biology and Cellular Response antibody; Cell Death antibody; Metabolism antibody; Neuroscience antibody |
| 预测分子量 | 68 kDa |
| 翻译后修饰 | Proteolytic cleavage by activated CASP3 leads to degradation and may regulate autophagy upon cellular stress and apoptotic stimuli. Phosphorylation at Ser-139 promotes association with the ATG12-ATG5 conjugate to form the ATG12-ATG5-ATG16L1 complex. |
检测图片 (3) Click the Picture to Zoom In
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ARG54957 anti-ATG16L antibody ICC/IF image
Immunofluorescence: U251 cells stained with ARG54957 anti-ATG16L antibody (green) at 1:25 dilution. DAPI (blue) for nuclear staining.
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ARG54957 anti-ATG16L antibody IHC-P image
Immunohistochemistry: Formalin-fixed and paraffin-embedded Human colon carcinoma tissue stained with ARG54957 anti-ATG16L antibody.
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ARG54957 anti-ATG16L antibody WB image
Western blot: 35 µg of NCI-H460 cell lysate stained with ARG54957 anti-ATG16L antibody.
