ARG54812

Name: anti-ATG7 antibody
Brand: arigo Biolaboratories
SKU: ARG54812
Price: 399 USD
Availability: InStock
Rating: 5.0 (1 reviews)

anti-ATG7 antibody

Datasheet

参考文献2

订购信息

包装

价格 (USD)

100 μl

399
351

arigo in CHINA:
恩瑞科生物
☎ 400-886-0853,
0571-8708-1890

概述

产品描述	Rabbit Polyclonal antibody recognizes ATG7
反应物种	Hu
预测物种	Ms, Rat, Chk
应用	FACS, IHC-P, WB
宿主	Rabbit
克隆	Polyclonal
同位型	IgG
靶点名称	ATG7
抗原物种	Human
抗原	KLH-conjugated synthetic peptide corresponding to aa. 540-569 of Human APG7L.
偶联标记	Un-conjugated
別名	Ubiquitin-like modifier-activating enzyme ATG7; hAGP7; Autophagy-related protein 7; GSA7; Ubiquitin-activating enzyme E1-like protein; APG7-LIKE; APG7L; APG7-like; ATG12-activating enzyme E1 ATG7

应用说明

应用建议

应用	推荐稀释比
FACS	1:10 - 1:50
IHC-P	Assay-dependent
WB	1:1000

应用说明

* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

属性

形式	Liquid
纯化	Purification with Protein A and immunogen peptide.
缓冲液	PBS and 0.09% (W/V) Sodium azide
抗菌剂	0.09% (W/V) Sodium azide
存放说明	For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
注意事项	For laboratory research only, not for drug, diagnostic or other use.

生物信息

数据库连接	GeneID: 10533 Human ATG7 Swiss-port # O95352 Human Ubiquitin-like modifier-activating enzyme ATG7
基因名称	ATG7
全名	autophagy related 7
背景介绍	This gene was identified based on homology to Pichia pastoris GSA7 and Saccharomyces cerevisiae APG7. In the yeast, the protein appears to be required for fusion of peroxisomal and vacuolar membranes. The protein shows homology to the ATP-binding and catalytic sites of the E1 ubiquitin activating enzymes. [provided by RefSeq, Jan 2009]
生物功能	E1-like activating enzyme involved in the 2 ubiquitin-like systems required for cytoplasm to vacuole transport (Cvt) and autophagy. Activates ATG12 for its conjugation with ATG5 as well as the ATG8 family proteins for their conjugation with phosphatidylethanolamine. Both systems are needed for the ATG8 association to Cvt vesicles and autophagosomes membranes. Required for autophagic death induced by caspase-8 inhibition. Required for mitophagy which contributes to regulate mitochondrial quantity and quality by eliminating the mitochondria to a basal level to fulfill cellular energy requirements and preventing excess ROS production. Modulates p53/TP53 activity to regulate cell cycle and survival during metabolic stress. Plays also a key role in the maintenance of axonal homeostasis, the prevention of axonal degeneration, the maintenance of hematopoietic stem cells, the formation of Paneth cell granules, as well as in adipose differentiation. [UniProt]
细胞定位	Cytoplasm. Preautophagosomal structure. Note=Localizes also to discrete punctae along the ciliary axoneme and to the base of the ciliary axoneme
研究领域	Cancer antibody; Cell Biology and Cellular Response antibody; Cell Death antibody; Metabolism antibody; Signaling Transduction antibody
预测分子量	78 kDa
翻译后修饰	Acetylated by EP300.

检测图片 (3) Click the Picture to Zoom In

ARG54812 anti-ATG7 antibody WB image

Western blot: 30 µg of 293T cell lysate stained with ARG54812 anti-ATG7 antibody at 1:1000 dilution.
ARG54812 anti-ATG7 antibody IHC-P image

Immunohistochemistry: Formalin-fixed and paraffin-embedded Human liver tissue stained with ARG54812 anti-ATG7 antibody.
ARG54812 anti-ATG7 antibody FACS image

Flow Cytometry: HeLa cells stained with ARG54812 anti-ATG7 antibody (right histogram) or without primary antibody control (left histogram), followed by incubation with FITC labelled secondary antibody.