ARG22406

anti-Adiponectin antibody

anti-Adiponectin antibody for IHC-Formalin-fixed paraffin-embedded sections,Western blot and Mouse,Rat

publication_link 参考文献1

概述

产品描述 Rabbit Polyclonal antibody recognizes Adiponectin
反应物种 Ms, Rat
预测物种 Hu, Bov, Chk
应用 IHC-P, WB
宿主 Rabbit
克隆 Polyclonal
同位型 IgG
靶点名称 Adiponectin
抗原物种 Human
抗原 Synthetic peptide around 15 aa (N-terminus) of Human Adiponectin.
偶联标记 Un-conjugated
別名 Adipose most abundant gene transcript 1 protein; ADPN; APM-1; Gelatin-binding protein; APM1; ACDC; Adiponectin; apM-1; ACRP30; ADIPQTL1; GBP28; 30 kDa adipocyte complement-related protein; Adipocyte, C1q and collagen domain-containing protein; Adipocyte complement-related 30 kDa protein

应用说明

应用建议
应用 推荐稀释比
IHC-P1 µg/ml
WB1 - 4 µg/ml
应用说明 * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
阳性对照 WB: Rat brain cell lysate.

属性

形式 Liquid
纯化 Affinity purification with immunogen.
缓冲液 PBS and 0.02% Sodium azide.
抗菌剂 0.02% Sodium azide
浓度 1 mg/ml
存放说明 For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
注意事项 For laboratory research only, not for drug, diagnostic or other use.

生物信息

数据库连接

GeneID: 11450 Mouse ADIPOQ

GeneID: 246253 Rat ADIPOQ

Swiss-port # Q60994 Mouse Adiponectin

基因名称 ADIPOQ
全名 adiponectin, C1Q and collagen domain containing
背景介绍 This gene is expressed in adipose tissue exclusively. It encodes a protein with similarity to collagens X and VIII and complement factor C1q. The encoded protein circulates in the plasma and is involved with metabolic and hormonal processes. Mutations in this gene are associated with adiponectin deficiency. Multiple alternatively spliced variants, encoding the same protein, have been identified. [provided by RefSeq, Apr 2010]
生物功能 Important adipokine involved in the control of fat metabolism and insulin sensitivity, with direct anti-diabetic, anti-atherogenic and anti-inflammatory activities. Stimulates AMPK phosphorylation and activation in the liver and the skeletal muscle, enhancing glucose utilization and fatty-acid combustion. Antagonizes TNF-alpha by negatively regulating its expression in various tissues such as liver and macrophages, and also by counteracting its effects. Inhibits endothelial NF-kappa-B signaling through a cAMP-dependent pathway. May play a role in cell growth, angiogenesis and tissue remodeling by binding and sequestering various growth factors with distinct binding affinities, depending on the type of complex, LMW, MMW or HMW. [UniProt]
预测分子量 26 kDa
翻译后修饰 Hydroxylated Lys-33 was not identified in PubMed:16497731, probably due to poor representation of the N-terminal peptide in mass fingerprinting.
HMW complexes are more extensively glycosylated than smaller oligomers. Hydroxylation and glycosylation of the lysine residues within the collagene-like domain of adiponectin seem to be critically involved in regulating the formation and/or secretion of HMW complexes and consequently contribute to the insulin-sensitizing activity of adiponectin in hepatocytes (By similarity).
O-glycosylated. Not N-glycosylated. O-linked glycans on hydroxylysines consist of Glc-Gal disaccharides bound to the oxygen atom of post-translationally added hydroxyl groups. Sialylated to varying degrees depending on tissue. Thr-22 appears to be the major site of sialylation. Higher sialylation found in SGBS adipocytes than in HEK fibroblasts. Sialylation is not required neither for heterodimerization nor for secretion. Not sialylated on the glycosylated hydroxylysines. Desialylated forms are rapidly cleared from the circulation.

检测图片 (2) Click the Picture to Zoom In

  • ARG22406 anti-Adiponectin antibody IHC-P image

    Immunohistochemistry: Rat brain tissue stained with ARG22406 anti-Adiponectin antibody at 1 µg/ml dilution.

  • ARG22406 anti-Adiponectin antibody WB image

    Western blot: Rat brain lysates stained with ARG22406 anti-Adiponectin antibody at 1, 2, and 4 µg/ml dilution.

文献引用

Maternal Separation Enhances Anti-contractile Perivascular Adipose Tissue Function in Male Rats on a High Fat Diet.

IHC-P / Rat

Loria AS et al.
Am J Physiol Regul Integr Comp Physiol.,  (2018)

publication_link

 

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