ARG63834
anti-ApoJ / Clusterin antibody
anti-ApoJ / Clusterin antibody for ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human
Cancer antibody; Cell Biology and Cellular Response antibody; Immune System antibody; Metabolism antibody; Signaling Transduction antibody
概述
| 产品描述 | Goat Polyclonal antibody recognizes ApoJ / Clusterin |
|---|---|
| 反应物种 | Hu |
| 预测物种 | Dog |
| 应用 | ICC/IF, IHC-P, WB |
| 特异性 | This antibody is expected to recognise both reported isoforms (NP_001822.2 and NP_976084.1) |
| 宿主 | Goat |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | ApoJ / Clusterin |
| 抗原物种 | Human |
| 抗原 | C-EKALQEYRKKHREE |
| 偶联标记 | Un-conjugated |
| 別名 | Clusterin; Apolipoprotein J; SGP-2; SP-40; APOJ; ApoJbeta; CLU2; CLU1; NA1/NA2; TRPM2; CLI; SGP2; Apo-J; TRPM-2; ApoJalpha; Aging-associated gene 4 protein; APO-J; Complement cytolysis inhibitor a chain; Ku70-binding protein 1; Complement cytolysis inhibitor b chain; Testosterone-repressed prostate message 2; Complement-associated protein SP-40,40; KUB1; AAG4; Complement cytolysis inhibitor |
应用说明
| 应用建议 |
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| 应用说明 | WB: Recommend incubate at RT for 1h. IHC-P: Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0). * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
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| 阳性对照 | Human colon | ||||||||
| 实际分子量 | ~ 37 kDa |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Purified from goat serum by antigen affinity chromatography. |
| 缓冲液 | Tris saline (pH 7.3), 0.02% Sodium azide and 0.5% BSA. |
| 抗菌剂 | 0.02% Sodium azide |
| 稳定剂 | 0.5% BSA |
| 浓度 | 0.5 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 背景介绍 | The protein encoded by this gene is a secreted chaperone that can under some stress conditions also be found in the cell cytosol. It has been suggested to be involved in several basic biological events such as cell death, tumor progression, and neurodegenerative disorders. Alternate splicing results in both coding and non-coding variants.[provided by RefSeq, May 2011] |
| 研究领域 | Cancer antibody; Cell Biology and Cellular Response antibody; Immune System antibody; Metabolism antibody; Signaling Transduction antibody |
| 预测分子量 | 52 kDa |
| 翻译后修饰 | Isoform 1 is proteolytically cleaved on its way through the secretory system, probably within the Golgi lumen. Polyubiquitinated, leading to proteasomal degradation. Heavily N-glycosylated. About 30% of the protein mass is comprised of complex N-linked carbohydrate. |
检测图片 (3) Click the Picture to Zoom In
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ARG63834 anti-ApoJ / Clusterin antibody ICC/IF image
Immunofluorescence: Paraformaldehyde-fixed HeLa cells permeabilized with 0.15% Triton. Cells were stained with ARG63834 anti-ApoJ / Clusterin antibody (green) at 10 µg/ml for 1 hour. DAPI (blue) for nuclear staining. Negative control: Unimmunized goat IgG (green) at 10 µg/ml dilution.
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ARG63834 anti-ApoJ / Clusterin antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Human tonsil tissue. Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0). The tissue section was stained with ARG63834 anti-ApoJ / Clusterin antibody at 2.5 µg/ml dilution followed by AP-staining.
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ARG63834 anti-ApoJ / Clusterin antibody WB image
Western blot: 35 µg of Human colon lysate (in RIPA buffer) stained with ARG63834 anti-ApoJ / Clusterin antibody at 0.1 µg/ml dilution and incubated at RT for 1 hour.
