ARG54921
anti-BACE1 antibody
anti-BACE1 antibody for ELISA,ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse
Cell Biology and Cellular Response antibody; Neuroscience antibody
概述
| 产品描述 | Rabbit Polyclonal antibody recognizes BACE1 |
|---|---|
| 反应物种 | Hu, Ms |
| 应用 | ELISA, ICC/IF, IHC-P, WB |
| 宿主 | Rabbit |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | BACE1 |
| 抗原物种 | Human |
| 抗原 | Syntehtic peptide (17 aa) within the last 50 aa of Human BACE. |
| 偶联标记 | Un-conjugated |
| 別名 | Beta-site APP cleaving enzyme 1; BACE; Beta-secretase 1; Membrane-associated aspartic protease 2; HSPC104; Memapsin-2; ASP2; Asp 2; EC 3.4.23.46; Aspartyl protease 2; Beta-site amyloid precursor protein cleaving enzyme 1 |
应用说明
| 应用建议 |
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| 应用说明 | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||||||
| 阳性对照 | Human Brain Tissue Lysate |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Purified by affinity chromatography. |
| 缓冲液 | PBS and 0.02% Sodium azide |
| 抗菌剂 | 0.02% Sodium azide |
| 浓度 | 1 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | BACE1 |
| 全名 | beta-site APP-cleaving enzyme 1 |
| 背景介绍 | BACE Antibody: Accumulation of the amyloid-beta (Abeta) plaque in the cerebral cortex is a critical event in the pathogenesis of Alzheimer's disease. Abeta peptide is generated by proteolytic cleavage of the beta-amyloid protein precursor (APP) at beta- and gamma-sites by two proteases. APP is first cleaved by beta-secretase, producing a soluble derivative of the protein and a membrane anchored 99-amino acid carboxy-terminal fragment (C99). The C99 fragment serves as substrate for gamma-secretase to generate the 4 kDa amyloid-beta peptide, which is deposited in the brains of all suffers of Alzheimer's disease. The long-sought beta-secretase was recently identified by several groups independently and designated beta-site APP cleaving enzyme (BACE) and aspartyl protease 2 (Asp2). BACE/Asp2 is a novel transmembrane aspartic protease and colocalizes with APP. |
| 生物功能 | Responsible for the proteolytic processing of the amyloid precursor protein (APP). Cleaves at the N-terminus of the A-beta peptide sequence, between residues 671 and 672 of APP, leads to the generation and extracellular release of beta-cleaved soluble APP, and a corresponding cell-associated C-terminal fragment which is later released by gamma-secretase. [UniProt] |
| 产品亮点 | Related products: BACE1 antibodies; Anti-Rabbit IgG secondary antibodies; Related news: Beta-amyloid Peptide, the Dr Jekyll and Mr Hyde of Alzheimer’s Disease |
| 研究领域 | Cell Biology and Cellular Response antibody; Neuroscience antibody |
| 预测分子量 | 56 kDa |
| 翻译后修饰 | N-Glycosylated. Acetylated in the endoplasmic reticulum at Lys-126, Lys-275, Lys-279, Lys-285, Lys-299, Lys-300 and Lys-307. Acetylation by NAT8 and NAT8B is transient and deacetylation probably occurs in the Golgi. Acetylation regulates the maturation, the transport to the plasma membrane, the stability and the expression of the protein. Palmitoylation mediates lipid raft localization. |
检测图片 (4) Click the Picture to Zoom In
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ARG54921 anti-BACE1 antibody WB image
Western blot: human brain tissue lysate in the absence (A) or presence (B) of blocking peptide and in mouse 3T3 cell lysate (C) stained with ARG54921 anti-BACE1 antibody at 1 ug/ml dilution.
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ARG54921 anti-BACE1 antibody IHC-P image
Immunohistochemistry: Mouse Brain stained with ARG54921 anti-BACE1 antibody at 2.5 μg/ml dilution.
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ARG54921 anti-BACE1 antibody ICC/IF image
Immunocytochemistry: 3T3 cells stained with ARG54921 anti-BACE1 antibody at 10 ug/ml dilution.
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ARG54921 anti-BACE1 antibody ICC/IF image
Immunocytochemistry: 3T3/NIH cells stained with ARG54921 anti-BACE1 antibody at 10 ug/ml dilution.
