ARG63290

anti-BERP / RNF22 antibody

anti-BERP / RNF22 antibody for Western blot and Human,Mouse,Rat

Cell Biology and Cellular Response antibody

概述

产品描述 Goat Polyclonal antibody recognizes BERP / RNF22
反应物种 Hu, Ms, Rat
预测物种 Cow, Dog
应用 WB
特异性 Both variants (NP_006449.2; NP_150594.2;) represent the same protein. This antibody is expected to recognise all three human isoforms of this protein.
宿主 Goat
克隆 Polyclonal
同位型 IgG
靶点名称 BERP / RNF22
抗原物种 Human
抗原 AKREDSPGPEVQP-C
偶联标记 Un-conjugated
別名 BERP; RING finger protein 97; RNF97; HAC1; Brain-expressed RING finger protein; Tripartite motif-containing protein 3; RING finger protein 22; RNF22

应用说明

应用建议
应用 推荐稀释比
WB0.1 - 0.3 µg/ml
应用说明 WB: Recommend incubate at RT for 1h.
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

属性

形式 Liquid
纯化 Purified from goat serum by antigen affinity chromatography.
缓冲液 Tris saline (pH 7.3), 0.02% Sodium azide and 0.5% BSA.
抗菌剂 0.02% Sodium azide
稳定剂 0.5% BSA
浓度 0.5 mg/ml
存放说明 For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
注意事项 For laboratory research only, not for drug, diagnostic or other use.

生物信息

数据库连接

GeneID: 10612 Human TRIM3

GeneID: 55992 Mouse TRIM3

GeneID: 83616 Rat TRIM3

背景介绍 The protein encoded by this gene is a member of the tripartite motif (TRIM) family, also called the 'RING-B-box-coiled-coil' (RBCC) subgroup of RING finger proteins. The TRIM motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. This protein localizes to cytoplasmic filaments. It is similar to a rat protein which is a specific partner for the tail domain of myosin V, a class of myosins which are involved in the targeted transport of organelles. The rat protein can also interact with alpha-actinin-4. Thus it is suggested that this human protein may play a role in myosin V-mediated cargo transport. Alternatively spliced transcript variants encoding the same isoform have been identified. [provided by RefSeq, Jul 2008]
研究领域 Cell Biology and Cellular Response antibody
预测分子量 81 kDa

检测图片 (3) Click the Picture to Zoom In

  • ARG63290 anti-BERP / RNF22 antibody WB image

    Western Blot: Mouse Brain lysate (RIPA buffer, 30µg total protein per lane) stained with ARG63290 anti-BERP / RNF22 antibody at 0.05 µg/ml dilution.

  • ARG63290 anti-BERP / RNF22 antibody WB image

    Western Blot: 1). Mock transfection; 2) TRIM3 (RC211928) expressing plasmid transfected HEK293 cell lysate standed with ARG63290 anti-BERP / RNF22 antibody

  • ARG63290 anti-BERP / RNF22 antibody WB image

    Western blot: 35 µg of Human cerebellum (A) and Rat brain (B) lysates (in RIPA buffer) stained with ARG63290 anti-BERP / RNF22 antibody at 0.3 µg/ml dilution and incubated at RT for 1 hour.