ARG45584
anti-BRD4 antibody
anti-BRD4 antibody for Flow cytometry,ICC/IF,Western blot and Human,Mouse,Rat
概述
| 产品描述 | Rabbit Polyclonal antibody recognizes BRD4 |
|---|---|
| 反应物种 | Hu, Ms, Rat |
| 应用 | FACS, ICC/IF, WB |
| 宿主 | Rabbit |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | BRD4 |
| 抗原物种 | Human |
| 抗原 | Synthetic peptide corresponding to middle region of human BRD4. |
| 偶联标记 | Un-conjugated |
| 別名 | BRD4; Bromodomain Containing 4; HUNK1; FSHRG4; HUNKI; MCAP; CAP; Female Sterile Homeotic Related Gene 4; Mitotic Chromosome-Associated Protein; Bromodomain-Containing Protein 4; Chromosome-Associated Protein; Bromodomain-Containing 4; Protein HUNK1; CDLS6 |
应用说明
| 应用建议 |
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| 应用说明 | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||||
| 实际分子量 | 220 kDa |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Affinity purified |
| 缓冲液 | 0.2% Na2HPO4, 0.9% NaCl and 4% Trehalose. |
| 稳定剂 | 4% Trehalose |
| 浓度 | 0.5 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | BRD4 |
| 全名 | Bromodomain Containing 4 |
| 背景介绍 | The protein encoded by this gene is homologous to the murine protein MCAP, which associates with chromosomes during mitosis, and to the human RING3 protein, a serine/threonine kinase. Each of these proteins contains two bromodomains, a conserved sequence motif which may be involved in chromatin targeting. This gene has been implicated as the chromosome 19 target of translocation t(15;19)(q13;p13.1), which defines an upper respiratory tract carcinoma in young people. Two alternatively spliced transcript variants have been described. [provided by RefSeq, Jul 2008] |
| 生物功能 | Chromatin reader protein that recognizes and binds acetylated histones and plays a key role in transmission of epigenetic memory across cell divisions and transcription regulation. [UniProt] |
| 细胞定位 | Chromosome; Nucleus. [UniProt] |
| 预测分子量 | 152 kDa |
| 翻译后修饰 | Acetylation; Isopeptide bond; Phosphoprotein; Ubl conjugation. [UniProt] |
检测图片 (5) Click the Picture to Zoom In
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ARG45584 anti-BRD4 antibody ICC/IF image
Immunofluorescence: MCF-7 stained with ARG45584 anti-BRD4 antibody at 5 μg/ml dilution.
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ARG45584 anti-BRD4 antibody WB image
Western blot: HepG2 stained with ARG45584 anti-BRD4 antibody at 0.5 μg/ml dilution.
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ARG45584 anti-BRD4 antibody WB image
Western blot: NIH/3T3 stained with ARG45584 anti-BRD4 antibody at 0.5 μg/ml dilution.
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ARG45584 anti-BRD4 antibody WB image
Western blot: C6 stained with ARG45584 anti-BRD4 antibody at 0.5 μg/ml dilution.
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ARG45584 anti-BRD4 antibody FACS image
Flow Cytometry: A549 stained with ARG45584 anti-BRD4 antibody at 1 µg/10^6 cells dilution.
