ARG55935
anti-Bcl 2 antibody [124]
anti-Bcl 2 antibody [124] for Flow cytometry,ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human
Cancer antibody; Cell Biology and Cellular Response antibody; Cell Death antibody; Metabolism antibody; Signaling Transduction antibody; Apoptosis Marker antibody
概述
| 产品描述 | Mouse Monoclonal antibody [124] recognizes Bcl 2 |
|---|---|
| 反应物种 | Hu |
| 不反应物种 | Ms, Rat |
| 应用 | FACS, ICC/IF, IHC-P, WB |
| 宿主 | Mouse |
| 克隆 | Monoclonal |
| 克隆号 | 124 |
| 同位型 | IgG1, kappa |
| 靶点名称 | Bcl 2 |
| 抗原物种 | Human |
| 抗原 | Synthetic peptide around aa. 41-54 of Human Bcl-2 protein (GAAPAPGIFSSQPG-C). |
| 偶联标记 | Un-conjugated |
| 別名 | BCL2; BCL2 Apoptosis Regulator; PPP1R50; Bcl-2; Protein Phosphatase 1, Regulatory Subunit 50; Apoptosis Regulator Bcl-2; B-Cell CLL/Lymphoma 2; BCL2, Apoptosis Regulator |
应用说明
| 应用建议 |
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|---|---|---|---|---|---|---|---|---|---|---|---|
| 应用说明 | IHC-P: Antigen Retrieval: Boil tissue section in 1 mM EDTA buffer (pH 7.5-8.5) for 10-20 min, followed by cooling at RT for 20 min. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Purification with Protein G. |
| 缓冲液 | PBS (pH 7.4), 0.05% Sodium azide and 0.1 mg/ml BSA |
| 抗菌剂 | 0.05% Sodium azide |
| 稳定剂 | 0.1 mg/ml BSA |
| 浓度 | 0.2 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | BCL2 |
| 全名 | B-cell CLL/lymphoma 2 |
| 背景介绍 | This gene encodes an integral outer mitochondrial membrane protein that blocks the apoptotic death of some cells such as lymphocytes. Constitutive expression of BCL2, such as in the case of translocation of BCL2 to Ig heavy chain locus, is thought to be the cause of follicular lymphoma. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Feb 2016] |
| 生物功能 | Suppresses apoptosis in a variety of cell systems including factor-dependent lymphohematopoietic and neural cells. [UniProt] |
| 细胞定位 | Cytoplasm, Endoplasmic reticulum, Membrane, Mitochondrion, Mitochondrion outer membrane, Nucleus. [UniProt] |
| 产品亮点 | Related Antibody Duos and Panels: ARG30268 Apoptosis Marker Antibody Duo (Bcl2, Bax) ARG30269 Apoptosis Marker Antibody Duo (Bcl2, Bid) Related products: Bcl-2 antibodies; Bcl-2 Duos / Panels; Anti-Rabbit IgG secondary antibodies; Related news: Lymphoma |
| 研究领域 | Cancer antibody; Cell Biology and Cellular Response antibody; Cell Death antibody; Metabolism antibody; Signaling Transduction antibody; Apoptosis Marker antibody |
| 预测分子量 | 26 kDa |
| 翻译后修饰 | Phosphoprotein, Ubl conjugation. [UniProt] |
检测图片 (3) Click the Picture to Zoom In
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ARG55935 anti-Bcl 2 antibody [124] WB image
Western blot: 30 µg of MCF-7 cell lysate stained with ARG55935 anti-Bcl 2 antibody [124] at 1:200 dilution.
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ARG55935 anti-Bcl 2 antibody [124] FACS image
Flow Cytometry: Jurkat cells stained with ARG55935 anti-Bcl 2 antibody [124] (red) and isotype control antibody (green).
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ARG55935 anti-Bcl 2 antibody [124] IHC-P image
Immunohistochemistry: Human non-Hodgkin's lymphoma stained with ARG55935 anti-Bcl 2 antibody [124]. Note nuclear membrane and cytoplasmic staining.
客户反馈
Good
anti-Bcl 2 antibody [124]
Application：WB
Sample：MCF-7
Sample Loading Amount：30 µg
Primary Antibody Dilution Factor：1:200
Primary Antibody Incubation Time：overnight
Primary Antibody Incubation Temperature：4 ºC
