ARG65415
anti-Bcl 2 antibody [Bcl2/100/D5]
anti-Bcl 2 antibody [Bcl2/100/D5] for Flow cytometry,ICC/IF,IHC-Frozen sections,IHC-Formalin-fixed paraffin-embedded sections,Immunoprecipitation,Western blot and Human
Cancer antibody; Cell Biology and Cellular Response antibody; Cell Death antibody; Metabolism antibody; Signaling Transduction antibody; Apoptosis Marker antibody
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概述
| 产品描述 | Mouse Monoclonal antibody [Bcl-2/100/D5] recognizes Bcl 2 |
|---|---|
| 反应物种 | Hu |
| 不反应物种 | Ms |
| 应用 | FACS, ICC/IF, IHC-Fr, IHC-P, IP, WB |
| 特异性 | The clone Bcl2/100 recognizes Bcl2, a 26 kDa protooncogen with anti-apoptotic effect, expressed in outer mitochondrial membrane, endoplasmic reticulum and nuclear envelope. |
| 宿主 | Mouse |
| 克隆 | Monoclonal |
| 克隆号 | Bcl2/100/D5 |
| 同位型 | IgG1 |
| 靶点名称 | Bcl 2 |
| 抗原物种 | Human |
| 抗原 | Synthetic peptide corresponding to the amino acids 41-54 of human Bcl2 |
| 偶联标记 | Un-conjugated |
| 別名 | BCL2; BCL2 Apoptosis Regulator; PPP1R50; Bcl-2; Protein Phosphatase 1, Regulatory Subunit 50; Apoptosis Regulator Bcl-2; B-Cell CLL/Lymphoma 2; BCL2, Apoptosis Regulator |
应用说明
| 应用建议 |
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| 应用说明 | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Purified from cell culture supernatant by protein-A affinity chromatography. |
| 纯度 | > 95% (by SDS-PAGE) |
| 缓冲液 | PBS (pH 7.4) and 15 mM Sodium azide |
| 抗菌剂 | 15 mM Sodium azide |
| 浓度 | 1 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | BCL2 |
| 全名 | B-cell CLL/lymphoma 2 |
| 背景介绍 | This gene encodes an integral outer mitochondrial membrane protein that blocks the apoptotic death of some cells such as lymphocytes. Constitutive expression of BCL2, such as in the case of translocation of BCL2 to Ig heavy chain locus, is thought to be the cause of follicular lymphoma. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Feb 2016] |
| 生物功能 | Suppresses apoptosis in a variety of cell systems including factor-dependent lymphohematopoietic and neural cells. [UniProt] |
| 细胞定位 | Cytoplasm, Endoplasmic reticulum, Membrane, Mitochondrion, Mitochondrion outer membrane, Nucleus. [UniProt] |
| 产品亮点 | Related Antibody Duos and Panels: ARG30268 Apoptosis Marker Antibody Duo (Bcl2, Bax) ARG30269 Apoptosis Marker Antibody Duo (Bcl2, Bid) Related products: Bcl-2 antibodies; Bcl-2 Duos / Panels; Anti-Rabbit IgG secondary antibodies; Related news: Lymphoma |
| 研究领域 | Cancer antibody; Cell Biology and Cellular Response antibody; Cell Death antibody; Metabolism antibody; Signaling Transduction antibody; Apoptosis Marker antibody |
| 预测分子量 | 26 kDa |
| 翻译后修饰 | Phosphoprotein, Ubl conjugation. [UniProt] |
检测图片 (2) Click the Picture to Zoom In
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ARG65415 anti-Bcl 2 antibody [Bcl2/100/D5] WB image
Western blot: 40 µg of Jurkat cell lysate stained with ARG65415 anti-Bcl 2 antibody [Bcl2/100/D5] at 1:500 dilution.
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ARG65415 anti-Bcl 2 antibody [Bcl2/100/D5] IHC-P image
Immunohistochemistry: Paraffin-embedded Human placenta tissue stained with ARG65415 anti-Bcl 2 antibody [Bcl2/100/D5] at 10 µg/ml dilution.
客户反馈
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anti-Bcl 2 antibody [Bcl2/100/D5]
Application：WB
Sample：Jurkat cell lysate
Sample Loading Amount：30 µg
Primary Antibody Dilution Factor：1:1000
Primary Antibody Incubation Time：overnight
Primary Antibody Incubation Temperature：4 ºC
