ARG54027
anti-BiP / GRP78 antibody
anti-BiP / GRP78 antibody for ICC/IF,Western blot and Human,Rat
Controls and Markers antibody; ER Marker antibody; Endoplasmic reticulum antibody
参考文献5
概述
| 产品描述 | Mouse Monoclonal antibody recognizes BiP / GRP78 |
|---|---|
| 反应物种 | Hu, Rat |
| 应用 | ICC/IF, WB |
| 宿主 | Mouse |
| 克隆 | Monoclonal |
| 同位型 | IgG |
| 靶点名称 | BiP / GRP78 |
| 抗原物种 | Human |
| 抗原 | Purified recombinant human BiP/GRP78 protein fragments expressed in E.coli. |
| 偶联标记 | Un-conjugated |
| 別名 | Heat shock 70 kDa protein 5; BiP; GRP-78; Endoplasmic reticulum lumenal Ca; MIF2; 78 kDa glucose-regulated protein; GRP78; Immunoglobulin heavy chain-binding protein; HEL-S-89n; BIP; 2+ |
应用说明
| 应用建议 |
|
||||||
|---|---|---|---|---|---|---|---|
| 应用说明 | IHC-P: Antigen Retrieval: Pressure cooking in Citrate buffer (pH 6.0). * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Affinity purified |
| 缓冲液 | PBS (pH 7.4), 0.02% Sodium azide and 50% Glycerol |
| 抗菌剂 | 0.02% Sodium azide |
| 稳定剂 | 50% Glycerol |
| 浓度 | 0.8 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | HSPA5 |
| 全名 | heat shock 70kDa protein 5 (glucose-regulated protein, 78kDa) |
| 背景介绍 | Probably plays a role in facilitating the assembly of multimeric protein complexes inside the ER |
| 生物功能 | Probably plays a role in facilitating the assembly of multimeric protein complexes inside the endoplasmic reticulum. Involved in the correct folding of proteins and degradation of misfolded proteins via its interaction with DNAJC10, probably to facilitate the release of DNAJC10 from its substrate. [UniProt] |
| 细胞定位 | Endoplasmic reticulum lumen. Melanosome |
| 研究领域 | Controls and Markers antibody; ER Marker antibody; Endoplasmic reticulum antibody |
| 预测分子量 | 72 kDa |
检测图片 (2) Click the Picture to Zoom In
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ARG54027 anti-BiP / GRP78 antibody WB image
Western blot: 30 µg of HeLa cell lysate stained with ARG54027 anti-BiP / GRP78 antibody at 1:1000 dilution.
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ARG54027 anti-BiP / GRP78 antibody ICC/IF image
Immunofluorescence: HeLa cells fixed with 4% Paraformaldehyde and stained with ARG54027 anti-BiP / GRP78 antibody at 1:50 dilution.
客户反馈
Good
anti-BiP / GRP78 antibody
Application：WB
Sample：HeLa
Sample Loading Amount：30 µg
Primary Antibody Dilution Factor：1:1000
Primary Antibody Incubation Time：overnight
Primary Antibody Incubation Temperature：4 ºC
文献引用
Endoplasmic reticulum stress promotes sorafenib resistance via miR-188-5p/hnRNPA2B1-mediated upregulation of PKM2 in hepatocellular carcinoma
WB / Human
Study of isolation and characterization of extracellular vesicles in senescent tumor cells induced by chemotherapeutics.
WB / Human
Melatonin Increases the Sensitivity of Hepatocellular Carcinoma to Sorafenib through the PERK-ATF4-Beclin1 Pathway
WB, ICC/IF / Human
Effects of calpain inhibitor on the apoptosis of hepatic stellate cells induced by calcium ionophore A23187.
WB / Human
Effects of calcium Ionophore A23187 on the apoptosis of hepatic stellate cells stimulated by transforming growth factor-β1.
WB / Rat
