ARG56171
anti-C4d / Complement 4d antibody [C4D204]
anti-C4d / Complement 4d antibody [C4D204] for IHC-Formalin-fixed paraffin-embedded sections and Human
概述
| 产品描述 | Mouse Monoclonal antibody [C4D204] recognizes C4d / Complement 4d |
|---|---|
| 反应物种 | Hu |
| 应用 | IHC-P |
| 宿主 | Mouse |
| 克隆 | Monoclonal |
| 克隆号 | C4D204 |
| 同位型 | IgG1, kappa |
| 靶点名称 | C4d / Complement 4d |
| 抗原物种 | Human |
| 抗原 | Human Complement 4d / c4d protein. |
| 偶联标记 | Un-conjugated |
| 別名 | Complement C4-A; C4AD; CPAMD2; CO4; Acidic complement C4; C4S; C4A6; C4A4; RG; C4A2; C4A3; C3 and PZP-like alpha-2-macroglobulin domain-containing protein 2; C4 |
应用说明
| 应用建议 |
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| 应用说明 | IHC-P: Antigen Retrieval: Boil tissue sections in 1 mM EDTA (pH 7.5-8.5) for 10-20 min, followed by cooling at RT for 20 min. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Purification with Protein G. |
| 缓冲液 | PBS (pH 7.4), 0.05% Sodium azide and 0.1 mg/ml BSA. |
| 抗菌剂 | 0.05% Sodium azide |
| 稳定剂 | 0.1 mg/ml BSA |
| 浓度 | 0.2 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | C4A |
| 全名 | complement component 4A (Rodgers blood group) |
| 背景介绍 | This gene encodes the acidic form of complement factor 4, part of the classical activation pathway. The protein is expressed as a single chain precursor which is proteolytically cleaved into a trimer of alpha, beta, and gamma chains prior to secretion. The trimer provides a surface for interaction between the antigen-antibody complex and other complement components. The alpha chain is cleaved to release C4 anaphylatoxin, an antimicrobial peptide and a mediator of local inflammation. Deficiency of this protein is associated with systemic lupus erythematosus and type I diabetes mellitus. This gene localizes to the major histocompatibility complex (MHC) class III region on chromosome 6. Varying haplotypes of this gene cluster exist, such that individuals may have 1, 2, or 3 copies of this gene. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Nov 2014] |
| 生物功能 | Non-enzymatic component of C3 and C5 convertases and thus essential for the propagation of the classical complement pathway. Covalently binds to immunoglobulins and immune complexes and enhances the solubilization of immune aggregates and the clearance of IC through CR1 on erythrocytes. C4A isotype is responsible for effective binding to form amide bonds with immune aggregates or protein antigens, while C4B isotype catalyzes the transacylation of the thioester carbonyl group to form ester bonds with carbohydrate antigens. Derived from proteolytic degradation of complement C4, C4a anaphylatoxin is a mediator of local inflammatory process. It induces the contraction of smooth muscle, increases vascular permeability and causes histamine release from mast cells and basophilic leukocytes. [UniProt] |
| 细胞定位 | Intracytoplasmic vacuoles of endothelial cells; Secreted |
| 预测分子量 | 193 kDa |
| 翻译后修饰 | Prior to secretion, the single-chain precursor is enzymatically cleaved to yield non-identical chains alpha, beta and gamma. During activation, the alpha chain is cleaved by C1 into C4a and C4b, and C4b stays linked to the beta and gamma chains. Further degradation of C4b by C1 into the inactive fragments C4c and C4d blocks the generation of C3 convertase. The proteolytic cleavages often are incomplete so that many structural forms can be found in plasma. N- and O-glycosylated. O-glycosylated with a core 1 or possibly core 8 glycan. |
检测图片 (3) Click the Picture to Zoom In
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ARG56171 anti-C4d / Complement 4d antibody [C4D204] IHC-P image
Immunohistochemistry: Formalin-fixed and paraffin-embedded Human kidney transplant tissue (10X) stained with ARG56171 anti-C4d / Complement 4d antibody [C4D204].
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ARG56171 anti-C4d / Complement 4d antibody [C4D204] IHC-P image
Immunohistochemistry: Formalin-fixed and paraffin-embedded Human kidney transplant tissue (20X) stained with ARG56171 anti-C4d / Complement 4d antibody [C4D204].
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ARG56171 anti-C4d / Complement 4d antibody [C4D204] IHC-P image
Immunohistochemistry: Formalin-fixed and paraffin-embedded Human kidney transplant tissue (40X) stained with ARG56171 anti-C4d / Complement 4d antibody [C4D204].
