ARG56590
anti-CCL2 / MCP1 antibody [2.2-4H5-1A11]
anti-CCL2 / MCP1 antibody [2.2-4H5-1A11] for ELISA,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse,Cow
参考文献2
概述
| 产品描述 | Mouse Monoclonal antibody [2.2-4H5-1A11] recognizes CCL2 / MCP1 |
|---|---|
| 反应物种 | Hu, Ms, Cow |
| 应用 | ELISA, IHC-P, WB |
| 宿主 | Mouse |
| 克隆 | Monoclonal |
| 克隆号 | 2.2-4H5-1A11 |
| 同位型 | IgG1, kappa |
| 靶点名称 | CCL2 / MCP1 |
| 抗原物种 | Human |
| 抗原 | E.coli derived Recombinant Human CCL2 / MCP1. (QPDAINAPVT CCYNFTNRKI SVQRLASYRR ITSSKCPKEA VIFKTIVAKE ICADPKQKWV QDSMDHLDKQ TQTPKT) |
| 偶联标记 | Un-conjugated |
| 別名 | MCP1; Monocyte chemotactic and activating factor; MCAF; Monocyte chemotactic protein 1; Monocyte secretory protein JE; HSMCR30; Small-inducible cytokine A2; HC11; SMC-CF; GDCF-2; SCYA2; C-C motif chemokine 2; Monocyte chemoattractant protein 1; MCP-1 |
应用说明
| 应用建议 |
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| 应用说明 | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Purification with Protein A. |
| 缓冲液 | PBS (pH 7.2) |
| 浓度 | 1 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | CCL2 |
| 全名 | chemokine (C-C motif) ligand 2 |
| 背景介绍 | This gene is one of several cytokine genes clustered on the q-arm of chromosome 17. Chemokines are a superfamily of secreted proteins involved in immunoregulatory and inflammatory processes. The superfamily is divided into four subfamilies based on the arrangement of N-terminal cysteine residues of the mature peptide. This chemokine is a member of the CC subfamily which is characterized by two adjacent cysteine residues. This cytokine displays chemotactic activity for monocytes and basophils but not for neutrophils or eosinophils. It has been implicated in the pathogenesis of diseases characterized by monocytic infiltrates, like psoriasis, rheumatoid arthritis and atherosclerosis. It binds to chemokine receptors CCR2 and CCR4. [provided by RefSeq, Jul 2013] |
| 生物功能 | Chemotactic factor that attracts monocytes and basophils but not neutrophils or eosinophils. Augments monocyte anti-tumor activity. Has been implicated in the pathogenesis of diseases characterized by monocytic infiltrates, like psoriasis, rheumatoid arthritis or atherosclerosis. May be involved in the recruitment of monocytes into the arterial wall during the disease process of atherosclerosis. [UniProt] |
| 产品亮点 | Related products: MCP1 antibodies; MCP1 ELISA Kits; MCP1 Duos / Panels; Anti-Mouse IgG secondary antibodies; Related news: HMGB1 in inflammation Inflammatory Cytokines |
| 预测分子量 | 11 kDa (Human); 16 kDa (Mouse) |
| 翻译后修饰 | Processing at the N-terminus can regulate receptor and target cell selectivity. Deletion of the N-terminal residue converts it from an activator of basophil to an eosinophil chemoattractant. |
检测图片 (6) Click the Picture to Zoom In
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ARG56590 anti-CCL2 / MCP1 antibody [2.2-4H5-1A11] IHC-P image
Immunohistochemistry: Formalin-fixed and paraffin-embedded sections of Human breast invasive ductal carcinoma. The recommended ARG56590 anti-CCL2 / MCP1 antibody [2.2-4H5-1A11] concentration is 10.0 µg/ml with an overnight incubation at 4˚C. An HRP-labeled polymer detection system was used with a DAB chromogen. Antigen Retrieval: Boil tissue section in Sodium Citrate buffer (pH 6.0) followed by cooling at RT for 20 min.
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ARG56590 anti-CCL2 / MCP1 antibody [2.2-4H5-1A11] WB image
Western blot: 250 - 0.24 ng (left to right) of Human MCP-1 stained with ARG56590 anti-CCL2 / MCP1 antibody [2.2-4H5-1A11], under reducing conditions.
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ARG56590 anti-CCL2 / MCP1 antibody [2.2-4H5-1A11] IHC-P image
Immunohistochemistry: Formalin-fixed and paraffin-embedded sections of Human breast invasive ductal carcinoma. The recommended ARG56590 anti-CCL2 / MCP1 antibody [2.2-4H5-1A11] concentration is 10.0 µg/ml with an overnight incubation at 4˚C. An HRP-labeled polymer detection system was used with a DAB chromogen. Antigen Retrieval: Boil tissue section in Sodium Citrate buffer (pH 6.0) followed by cooling at RT for 20 min.
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ARG56590 anti-CCL2 / MCP1 antibody [2.2-4H5-1A11] IHC-P image
Immunohistochemistry: Formalin-fixed and paraffin-embedded sections of Human breast invasive ductal carcinoma. The recommended ARG56590 anti-CCL2 / MCP1 antibody [2.2-4H5-1A11] concentration is 10.0 µg/ml with an overnight incubation at 4˚C. An HRP-labeled polymer detection system was used with a DAB chromogen. Antigen Retrieval: Boil tissue section in Sodium Citrate buffer (pH 6.0) followed by cooling at RT for 20 min.
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ARG56590 anti-CCL2 / MCP1 antibody [2.2-4H5-1A11] WB image
Western blot: 250 - 0.24 ng (left to right) of Human MCP-1 stained with ARG56590 anti-CCL2 / MCP1 antibody [2.2-4H5-1A11], under non-reducing conditions.
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ARG56590 anti-CCL2 / MCP1 antibody [2.2-4H5-1A11] standard curve image
Sandwich ELISA: ARG56590 anti-CCL2 / MCP1 antibody [2.2-4H5-1A11] as a capture antibody at 2.0 - 4.0 µg/ml combined with ARG56731 anti-CCL2 / MCP1 antibody (Biotin) as a detection antibody at ~ 0.5 - 1.0 µg/ml. Results of a typical standard run with optical density.
文献引用
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