ARG56704
anti-CCL20 / MIP3 alpha antibody
anti-CCL20 / MIP3 alpha antibody for ELISA,IHC-Formalin-fixed paraffin-embedded sections,Neutralizing,Western blot and Human
概述
| 产品描述 | Rabbit Polyclonal antibody recognizes CCL20 / MIP3 alpha |
|---|---|
| 反应物种 | Hu |
| 应用 | ELISA, IHC-P, Neut, WB |
| 宿主 | Rabbit |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | CCL20 / MIP3 alpha |
| 抗原物种 | Human |
| 抗原 | E.coli derived Recombinant Human MIP-3α (CCL20). (ASNFDCCLGY TDRILHPKFI VGFTRQLANE GCDINAIIFH TKKKLSVCAN PKQTWVKYIV RLLSKKVKNM) |
| 偶联标记 | Un-conjugated |
| 別名 | ST38; CC chemokine LARC; Exodus; Macrophage inflammatory protein 3 alpha; 2-70; MIP-3a; Beta-chemokine exodus-1; SCYA20; MIP-3-alpha; LARC; MIP3A; 1-64; Small-inducible cytokine A20; 1-67; CKb4; C-C motif chemokine 20; Liver and activation-regulated chemokine |
应用说明
| 应用建议 |
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| 应用说明 | Neutralizing: The application for neutralizing is lot-specific, please contact info@arigobio.com for the detail. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Affinity purification with immunogen. |
| 缓冲液 | PBS (pH 7.2) |
| 浓度 | 1 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | CCL20 |
| 全名 | chemokine (C-C motif) ligand 20 |
| 背景介绍 | This antimicrobial gene belongs to the subfamily of small cytokine CC genes. Cytokines are a family of secreted proteins involved in immunoregulatory and inflammatory processes. The CC cytokines are proteins characterized by two adjacent cysteines. The protein encoded by this gene displays chemotactic activity for lymphocytes and can repress proliferation of myeloid progenitors. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2014] |
| 生物功能 | Chemotactic factor that attracts lymphocytes and, slightly, neutrophils, but not monocytes. Inhibits proliferation of myeloid progenitors in colony formation assays. May be involved in formation and function of the mucosal lymphoid tissues by attracting lymphocytes and dendritic cells towards epithelial cells. C-terminal processed forms have been shown to be equally chemotactically active for leukocytes. Possesses antibacterial activity E.coli ATCC 25922 and S.aureus ATCC 29213. [UniProt] |
| 预测分子量 | 11 kDa |
| 翻译后修饰 | C-terminal processed forms which lack 1, 3 or 6 amino acids are produced by proteolytic cleavage after secretion from peripheral blood monocytes. |
检测图片 (4) Click the Picture to Zoom In
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ARG56704 anti-CCL20 / MIP3 alpha antibody IHC-P image
Immunohistochemistry: Formalin-fixed and paraffin-embedded sections of Human colon/rectum adenocarcinoma tissue. The recommended ARG56704 anti-CCL20 / MIP3 alpha antibody concentration is 0.5 µg/ml with an overnight incubation at 4°C. An HRP-labeled polymer detection system was used with a DAB chromogen. Antigen Retrieval: Boil tissue section in Sodium Citrate buffer (pH 6.0) followed by cooling at RT for 20 min.
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ARG56704 anti-CCL20 / MIP3 alpha antibody WB image
Western blot: 250 - 0.24 ng of HumanMIP-3α stained with ARG56704 anti-CCL20 / MIP3 alpha antibody, under reducing conditions.
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ARG56704 anti-CCL20 / MIP3 alpha antibody WB image
Western blot: 250 - 0.24 ng of HumanMIP-3α stained with ARG56704 anti-CCL20 / MIP3 alpha antibody, under non-reducing conditions.
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ARG56704 anti-CCL20 / MIP3 alpha antibody standard curve image
Sandwich ELISA: ARG56704 anti-CCL20 / MIP3 alpha antibody as a capture antibody at 0.5 - 2.0 µg/ml combined with ARG56813 anti-CCL20 antibody (Biotin) as a detection antibody. Results of a typical standard run with optical density.
