ARG56709

anti-CCL8 / MCP2 antibody

Datasheet

参考文献1

订购信息

包装

价格 (USD)

50 μg

335

arigo in CHINA:
恩瑞科生物
☎ 400-886-0853,
0571-8708-1890

概述

产品描述	Rabbit Polyclonal antibody recognizes CCL8 / MCP2
反应物种	Hu
应用	ELISA, IHC-P, WB
宿主	Rabbit
克隆	Polyclonal
同位型	IgG
靶点名称	CCL8 / MCP2
抗原物种	Human
抗原	E.coli derived Recombinant Human MCP-2 (CCL8). (QPDSVSIPIT CCFNVINRKI PIQRLESYTR ITNIQCPKEA VIFKTKRGKE VCADPKERWV RDSMKHLDQI FQNLKP)
偶联标记	Un-conjugated
別名	SCYA10; MCP2; Small-inducible cytokine A8; 6-76; Monocyte chemotactic protein 2; HC14; SCYA8; C-C motif chemokine 8; MCP-2; Monocyte chemoattractant protein 2

应用说明

应用建议

应用	推荐稀释比
ELISA	Sandwich: 0.5 - 2.0 µg/ml with ARG56818 as a detection antibody
IHC-P	0.75 µg/ml
WB	0.1 - 0.2 µg/ml

应用说明

* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

属性

形式	Liquid
纯化	Affinity purification with immunogen.
缓冲液	PBS (pH 7.2)
浓度	1 mg/ml
存放说明	For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
注意事项	For laboratory research only, not for drug, diagnostic or other use.

生物信息

数据库连接	GeneID: 6355 Human CCL8 Swiss-port # P80075 Human C-C motif chemokine 8
基因名称	CCL8
全名	chemokine (C-C motif) ligand 8
背景介绍	This antimicrobial gene is one of several chemokine genes clustered on the q-arm of chromosome 17. Chemokines form a superfamily of secreted proteins involved in immunoregulatory and inflammatory processes. The superfamily is divided into four subfamilies based on the arrangement of N-terminal cysteine residues of the mature peptide. This chemokine is a member of the CC subfamily which is characterized by two adjacent cysteine residues. This cytokine displays chemotactic activity for monocytes, lymphocytes, basophils and eosinophils. By recruiting leukocytes to sites of inflammation this cytokine may contribute to tumor-associated leukocyte infiltration and to the antiviral state against HIV infection. [provided by RefSeq, Sep 2014]
生物功能	Chemotactic factor that attracts monocytes, lymphocytes, basophils and eosinophils. May play a role in neoplasia and inflammatory host responses. This protein can bind heparin. The processed form MCP-2(6-76) does not show monocyte chemotactic activity, but inhibits the chemotactic effect most predominantly of CCL7, and also of CCL2 and CCL5 and CCL8. [UniProt]
预测分子量	11 kDa
翻译后修饰	N-terminal processed form MCP-2(6-76) is produced by proteolytic cleavage after secretion from peripheral blood monocytes.

检测图片 (7) Click the Picture to Zoom In

ARG56709 anti-CCL8 / MCP2 antibody IHC-P image

Immunohistochemistry: Formalin-fixed and paraffin-embedded sections of Human normal gastric mucosa. The recommended ARG56709 anti-CCL8 / MCP2 antibody concentration is 0.75 µg/ml with an overnight incubation at 4˚C. An HRP-labeled polymer detection system was used with a DAB chromogen. Antigen Retrieval: Boil tissue section in Sodium Citrate buffer (pH 6.0) followed by cooling at RT for 20 min.
ARG56709 anti-CCL8 / MCP2 antibody WB image

Western blot: 250 - 0.24 ng of Human MCP-2 stained with ARG56709 anti-CCL8 / MCP2 antibody, under reducing conditions.
ARG56709 anti-CCL8 / MCP2 antibody WB image

Western blot: 250 - 0.24 ng of Human MCP-2 stained with ARG56709 anti-CCL8 / MCP2 antibody, under reducing conditions.
ARG56709 anti-CCL8 / MCP2 antibody IHC-P image

Immunohistochemistry: Formalin-fixed and paraffin-embedded sections of Human normal gastric mucosa. The recommended ARG56709 anti-CCL8 / MCP2 antibody concentration is 0.75 µg/ml with an overnight incubation at 4˚C. An HRP-labeled polymer detection system was used with a DAB chromogen. Antigen Retrieval: Boil tissue section in Sodium Citrate buffer (pH 6.0) followed by cooling at RT for 20 min.
ARG56709 anti-CCL8 / MCP2 antibody WB image

Western blot: 250 - 0.24 ng of Human MCP-2 stained with ARG56709 anti-CCL8 / MCP2 antibody, under non-reducing conditions.
ARG56709 anti-CCL8 / MCP2 antibody WB image

Western blot: 250 - 0.24 ng of Human MCP-2 stained with ARG56709 anti-CCL8 / MCP2 antibody, under non-reducing conditions.
ARG56709 anti-CCL8 / MCP2 antibody standard curve image

Sandwich ELISA: ARG56709 anti-CCL8 / MCP2 antibody as a capture antibody at 0.5 - 2.0 µg/ml combined with ARG56818 anti-MCP2 / CCL8 antibody (Biotin) as a detection antibody. Results of a typical standard run with optical density.