ARG65453
anti-CD107a / LAMP1 antibody [H4A3] (azide free)
anti-CD107a / LAMP1 antibody [H4A3] (azide free) for Electron microscopy,Flow cytometry,ICC/IF,IHC-Frozen sections,IHC-Formalin-fixed paraffin-embedded sections,Immunoprecipitation,Purification,Western blot and Human,Mouse,Rat,Primates
Cancer antibody; Cell Death antibody; Controls and Markers antibody; Developmental Biology antibody; Metabolism antibody; Neuroscience antibody; Signaling Transduction antibody; Lysosome Marker antibody
概述
| 产品描述 | Azide free Mouse Monoclonal antibody [H4A3] recognizes CD107a / LAMP1 |
|---|---|
| 反应物种 | Hu, Ms, Rat, NHuPrm |
| 应用 | EM, FACS, ICC/IF, IHC-Fr, IHC-P, IP, Puri, WB |
| 特异性 | The mouse monoclonal antibody H4A3 recognizes LAMP1 / CD107a, an approximately 100120 kDa glycoprotein expressed mainly on lysosomal, but also on the plasma membrane. |
| 宿主 | Mouse |
| 克隆 | Monoclonal |
| 克隆号 | H4A3 |
| 同位型 | IgG1 |
| 靶点名称 | CD107a / LAMP1 |
| 抗原物种 | Human |
| 抗原 | Human PBMC |
| 偶联标记 | Un-conjugated |
| 別名 | LGP120; CD107a; LAMPA; CD antigen CD107a; Lysosome-associated membrane glycoprotein 1; CD107 antigen-like family member A; LAMP-1; Lysosome-associated membrane protein 1 |
应用说明
| 应用建议 |
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| 应用说明 | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Purified from cell culture supernatant by protein-A affinity chromatography. |
| 纯度 | > 95% (by SDS-PAGE) |
| 缓冲液 | BBS (pH 8.2) |
| 浓度 | 0.1 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | LAMP1 |
| 全名 | lysosomal-associated membrane protein 1 |
| 背景介绍 | CD107a (lysosome-associated membrane protein-1, LAMP-1), together with LAMP-2, is a major constituent of lysosomal membrane, 1-2% of total CD107a is found also on the plasma membrane. The LAMP proteins are involved in lysosome biogenesis and are required for fusion of lysosomes with phagosomes. Increased CD107a immunoreactivity is observed in neurones, and in glial cells surrounding senile plaques in Alzheimers disease cases and is localized mainly in medullary epithelial cells, single macrophages and lymphocytes in acute thymic involution. CD107a is a good marker of mast cell activation. |
| 生物功能 | Presents carbohydrate ligands to selectins. Also implicated in tumor cell metastasis. [UniProt] |
| 产品亮点 | Related products: LAMP1 antibodies; LAMP1 Duos / Panels; Anti-Mouse IgG secondary antibodies; Related news: Baking soda restores circadian clock in tumor cells |
| 研究领域 | Cancer antibody; Cell Death antibody; Controls and Markers antibody; Developmental Biology antibody; Metabolism antibody; Neuroscience antibody; Signaling Transduction antibody; Lysosome Marker antibody |
| 预测分子量 | 45 kDa |
| 翻译后修饰 | O- and N-glycosylated; some of the 18 N-linked glycans are polylactosaminoglycans. The glycosylation of N-76 is essential for Lassa virus entry into cells. |
检测图片 (2) Click the Picture to Zoom In
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ARG65453 anti-CD107a / LAMP1 antibody [H4A3] (azide free) ICC/IF image
Immunofluorescence: 100% Methanol fixed (RT, 10 min) HeLa cells stained with ARG65453 anti-CD107a / LAMP1 antibody [H4A3] (azide free) at 1:50 dilution.
Secondary antibody: ARG55393 Goat anti-Mouse IgG (H+L) antibody (FITC)
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ARG65453 anti-CD107a / LAMP1 antibody [H4A3] (azide free) WB image
Western blot: 30 µg of HeLa cell lysate stained with ARG65453 anti-CD107a / LAMP1 antibody [H4A3] (azide free) at 1:1000 dilution.
客户反馈
Excellent
anti-CD107a / LAMP1 antibody [H4A3] (azide free)
Application：IF/ICC
Sample：HeLa
Fixation Buffer：100% Methanol
Fixation Time：10 min
Fixation Temperature：20 - 25 ºC
Permeabilization Buffer：0.1% Triton X-100
Primary Antibody Dilution Factor：1:50
Primary Antibody Incubation Time：overnight
Primary Antibody Incubation Temperature：4 ºC
Conjugation of Secondary Antibody：FITC
Excellent
anti-CD107a / LAMP1 antibody [H4A3] (azide free)
Application：WB
Sample：HeLa
Sample Loading Amount：30 µg
Primary Antibody Dilution Factor：1:1000
Primary Antibody Incubation Time：overnight
Primary Antibody Incubation Temperature：4 ºC
克隆号文献
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