ARG43342
anti-CD107b / LAMP2b antibody
anti-CD107b / LAMP2b antibody for ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse
概述
| 产品描述 | Rabbit Polyclonal antibody recognizes CD107b / LAMP2b |
|---|---|
| 反应物种 | Hu, Ms |
| 应用 | ICC/IF, IHC-P, WB |
| 特异性 | The antibody reacts to LAMP2b isoform and has a cross-reactivity to LAMP2c isoform (64% homology). |
| 宿主 | Rabbit |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | CD107b / LAMP2b |
| 抗原物种 | Human |
| 抗原 | A 17-amino acid synthetic peptide within the last 50 amino acids of Human CD107b / LAMP2b isoform. |
| 偶联标记 | Un-conjugated |
| 別名 | CD antigen CD107b; LAMPB; CD107b; LGP110; Lysosome-associated membrane glycoprotein 2; LAMP-2; CD107 antigen-like family member B; Lysosome-associated membrane protein 2 |
应用说明
| 应用建议 |
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| 应用说明 | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||||
| 阳性对照 | HepG2 | ||||||||
| 实际分子量 | 100 ~ 120 kDa (glycosylated) |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Affinity purification with immunogen. |
| 缓冲液 | PBS and 0.02% Sodium azide. |
| 抗菌剂 | 0.02% Sodium azide |
| 浓度 | 1 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 |
Swiss-port # P13473 Human Lysosome-associated membrane glycoprotein 2 Swiss-port # P17047 Mouse Lysosome-associated membrane glycoprotein 2 |
|---|---|
| 基因名称 | LAMP2 |
| 全名 | lysosomal-associated membrane protein 2 |
| 背景介绍 | The protein encoded by this gene is a member of a family of membrane glycoproteins. This glycoprotein provides selectins with carbohydrate ligands. It may play a role in tumor cell metastasis. It may also function in the protection, maintenance, and adhesion of the lysosome. Alternative splicing of this gene results in multiple transcript variants encoding distinct proteins. [provided by RefSeq, Jul 2008] |
| 生物功能 | Plays an important role in chaperone-mediated autophagy, a process that mediates lysosomal degradation of proteins in response to various stresses and as part of the normal turnover of proteins with a long biological half-live (PubMed:8662539, PubMed:11082038, PubMed:18644871, PubMed:24880125, PubMed:27628032). Functions by binding target proteins, such as GAPDH and MLLT11, and targeting them for lysosomal degradation (PubMed:8662539, PubMed:11082038, PubMed:18644871, PubMed:24880125). Plays a role in lysosomal protein degradation in response to starvation (By similarity). Required for the fusion of autophagosomes with lysosomes during autophagy (PubMed:27628032). Cells that lack LAMP2 express normal levels of VAMP8, but fail to accumulate STX17 on autophagosomes, which is the most likely explanation for the lack of fusion between autophagosomes and lysosomes (PubMed:27628032). Required for normal degradation of the contents of autophagosomes (PubMed:27628032). Required for efficient MHCII-mediated presentation of exogenous antigens via its function in lysosomal protein degradation; antigenic peptides generated by proteases in the endosomal/lysosomal compartment are captured by nascent MHCII subunits (PubMed:20518820). Is not required for efficient MHCII-mediated presentation of endogenous antigens (PubMed:20518820). [Isoform LAMP-2C]: Modulates chaperone-mediated autophagy. Decreases presentation of endogenous antigens by MHCII. Does not play a role in the presentation of exogenous and membrane-derived antigens by MHCII. [UniProt] |
| 细胞定位 | Cell membrane; Single-pass type I membrane protein. Endosome membrane; Single-pass type I membrane protein. Lysosome membrane; Single-pass type I membrane protein. Cytoplasmic vesicle, autophagosome membrane. Note=This protein shuttles between lysosomes, endosomes, and the plasma membrane. [UniProt] |
| 预测分子量 | 45 kDa |
| 翻译后修饰 | O- and N-glycosylated; some of the 16 N-linked glycans are polylactosaminoglycans. [UniProt] |
检测图片 (3) Click the Picture to Zoom In
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ARG43342 anti-CD107b / LAMP2b antibody ICC image
Immunocytochemistry: HepG2 cells stained with ARG43342 anti-CD107b / LAMP2b antibody at 10 µg/ml dilution.
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ARG43342 anti-CD107b / LAMP2b antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Mouse liver tissue stained with ARG43342 anti-CD107b / LAMP2b antibody at 5 µg/ml dilution.
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ARG43342 anti-CD107b / LAMP2b antibody WB image
Western blot: HepG2 cell lysate stained with ARG43342 anti-CD107b / LAMP2b antibody at 1 µg/ml (left) and 2 µg/ml (right) dilution.
