ARG65394
anti-CD116 antibody [4H1]
anti-CD116 antibody [4H1] for CyTOF®-candidate,Flow cytometry,IHC-Frozen sections,Immunoprecipitation,Western blot and Human
Developmental Biology antibody; Immune System antibody
概述
| 产品描述 | Mouse Monoclonal antibody [4H1] recognizes CD116 |
|---|---|
| 反应物种 | Hu |
| 应用 | CyTOF®-candidate, FACS, IHC-Fr, IP, WB |
| 特异性 | The clone 4H1 recognizes human CD116, the GM-CSF receptor alpha subunit (approx. 80 kDa) expressed e.g. by neutrophils, eosinophils, monocytes and macrophages. |
| 宿主 | Mouse |
| 克隆 | Monoclonal |
| 克隆号 | 4H1 |
| 同位型 | IgG1 |
| 靶点名称 | CD116 |
| 抗原 | CD116-transfected COS cells |
| 偶联标记 | Un-conjugated |
| 別名 | Granulocyte-macrophage colony-stimulating factor receptor subunit alpha; GMCSFR; CDw116; GM-CSF-R-alpha; CD antigen CD116; CSF2R; CD116; GMR-alpha; CSF2RAX; CSF2RAY; GMCSFR-alpha; GMR; SMDP4; CSF2RX; CSF2RY |
应用说明
| 应用建议 |
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| 应用说明 | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Purified from cell culture supernatant by protein-A affinity chromatography. |
| 纯度 | > 95% (by SDS-PAGE) |
| 缓冲液 | TBS (pH 8.0) and 15 mM Sodium azide |
| 抗菌剂 | 15 mM Sodium azide |
| 浓度 | 1 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 |
Swiss-port # P15509 Human Granulocyte-macrophage colony-stimulating factor receptor subunit alpha |
|---|---|
| 基因名称 | CSF2RA |
| 全名 | colony stimulating factor 2 receptor, alpha, low-affinity (granulocyte-macrophage) |
| 背景介绍 | CD116 (GM-CSF R alpha) is the low affinity receptor for granulocyte-macrophage colony-stimulating factor (GM-CSF). CD116 heterodimerizes with CD131, the common beta chain subunit shared with IL-3 and IL5 receptors, to form the high affinity GM-CSF receptor. CD116 is expressed by myeloid cells including macrophages, neutrophils, eosinophils, dendritic cells, and their precursors, as well as on endothelial cells. It is being used as a specific marker of myeloid leukemias. |
| 生物功能 | Low affinity receptor for granulocyte-macrophage colony-stimulating factor. Transduces a signal that results in the proliferation, differentiation, and functional activation of hematopoietic cells. [UniProt] |
| 产品亮点 | Related products: CD116 antibodies; CD116 ELISA Kits; Anti-Mouse IgG secondary antibodies; Related news: CyTOF-candidate Antibodies |
| 研究领域 | Developmental Biology antibody; Immune System antibody |
| 预测分子量 | 46 kDa |
检测图片 (2) Click the Picture to Zoom In
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ARG65394 anti-CD116 antibody [4H1] FACS image
Flow Cytometry: Human peripheral blood stained with ARG65394 anti-CD116 antibody [4H1] at 3 µg/ml dilution, followed by PE-conjugated Goat anti-Mouse antibody.
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ARG65394 anti-CD116 antibody [4H1] FACS image
Flow Cytometry: Separation of human monocytes (red-filled) from lymphocytes (black-dashed). Human peripheral whole blood stained with ARG65394 anti-CD116 antibody [4H1] at 3 µg/ml dilution, followed by PE-conjugated Goat anti-Mouse antibody.
客户反馈
Average
anti-CD116 antibody [4H1]
Application：WB
Sample：293T
Sample Loading Amount：20 µg
Primary Antibody Dilution Factor：1:300
Primary Antibody Incubation Time：overnight
Primary Antibody Incubation Temperature：4 ºC
