ARG23456
anti-CD11b + CD11c antibody [OX-42]
anti-CD11b + CD11c antibody [OX-42] for Flow cytometry,ICC/IF,IHC-Frozen sections,Immunoprecipitation and Rat
MDSC Marker antibody; Myeloid-derived suppressor cell antibody
概述
| 产品描述 | Mouse Monoclonal antibody [OX-42] recognizes CD11b + CD11c Mouse anti Rat CD11b, clone OX-42, recognizes rat CD11b, also known as integrin alpha-M, the receptor for the iC3b component of complement. CD11b is a 1151 amino acid single pass type 1 transmembrane glycoprotein posessing a single vWFA domain and multiple FG-GAP repeats. CD11b is expressed on most macrophages, including resident and activated peritoneal macrophages and Kupffer cells and around 35% of alveolar macrophages. The antibody also labels dendritic cells, granulocytes and microglia in the brain (Robinson et al.1986).Mouse anti Rat CD11b, clone OX-42 is reported to inhibit complement mediated rosettes (Robinson et al.1986) as well as inhibit myelin binding and uptake (van der Laan et al.1996). |
|---|---|
| 反应物种 | Rat |
| 应用 | FACS, ICC/IF, IHC-Fr, IP |
| 宿主 | Mouse |
| 克隆 | Monoclonal |
| 克隆号 | OX-42 |
| 同位型 | IgG2a |
| 靶点名称 | CD11b + CD11c |
| 抗原物种 | Rat |
| 抗原 | Resident rat peritoneal macrophages. |
| 偶联标记 | Un-conjugated |
| 別名 | MAC1A; CR3A; CR-3 alpha chain; Cell surface glycoprotein MAC-1 subunit alpha; Integrin alpha-M; MAC-1; CD11 antigen-like family member B; Leukocyte adhesion receptor MO1; MO1A; SLEB6; Neutrophil adherence receptor; CD antigen CD11b; CD11B |
应用说明
| 应用建议 |
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| 应用说明 | FACS: Use 10 µl of the suggested working dilution to label 10^6 cells in 100 µl. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Purification with Protein G. |
| 缓冲液 | PBS and 0.09% Sodium azide. |
| 抗菌剂 | 0.09% Sodium azide |
| 浓度 | 1 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | ITGAM |
| 全名 | integrin, alpha M (complement component 3 receptor 3 subunit) |
| 背景介绍 | CD11b (integrin alpha M chain): Integrins are heterodimeric integral membrane proteins composed of an alpha chain and a beta chain. This I-domain containing alpha integrin combines with the beta 2 chain (ITGB2) to form a leukocyte-specific integrin referred to as macrophage receptor 1 ('Mac-1'), or inactivated-C3b (iC3b) receptor 3 ('CR3'). The alpha M beta 2 integrin is important in the adherence of neutrophils and monocytes to stimulated endothelium, and also in the phagocytosis of complement coated particles. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Mar 2009] |
| 生物功能 | CD11b: Integrin ITGAM/ITGB2 is implicated in various adhesive interactions of monocytes, macrophages and granulocytes as well as in mediating the uptake of complement-coated particles and pathogens (PubMed:9558116, PubMed:20008295). It is identical with CR-3, the receptor for the iC3b fragment of the third complement component. It probably recognizes the R-G-D peptide in C3b. Integrin ITGAM/ITGB2 is also a receptor for fibrinogen, factor X and ICAM1. It recognizes P1 and P2 peptides of fibrinogen gamma chain. Regulates neutrophil migration (PubMed:28807980). In association with beta subunit ITGB2/CD18, required for CD177-PRTN3-mediated activation of TNF primed neutrophils (PubMed:21193407). May regulate phagocytosis-induced apoptosis in extravasated neutrophils. May play a role in mast cell development. Required with TYROBP/DAP12 in microglia to control production of microglial superoxide ions which promote the neuronal apoptosis that occurs during brain development. [UniProt] |
| 产品亮点 | Related products: CD11 antibodies; CD11 ELISA Kits; CD11 Duos / Panels; Anti-Mouse IgG secondary antibodies; Related news: New antibody panels and duos for Tumor immune microenvironment |
| 研究领域 | MDSC Marker antibody; Myeloid-derived suppressor cell antibody |
| 预测分子量 | 127 kDa |
检测图片 (3) Click the Picture to Zoom In
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ARG23456 anti-CD11b + CD11c antibody [OX-42] IHC-Fr image
Immunohistochemistry: Rat lymph node cryosection stained with ARG23456 anti-CD11b + CD11c antibody [OX-42], red in A and Mouse anti Rat CD8, green in B. C is the merged image with nuclei counter-stained in blue using DAPI. (Low power).
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ARG23456 anti-CD11b + CD11c antibody [OX-42] IHC-Fr image
Immunohistochemistry: Rat lymph node cryosection stained with ARG23456 anti-CD11b + CD11c antibody [OX-42], red in A and Mouse anti Rat CD8, green in B. C is the merged image with nuclei counter-stained in blue using DAPI. (Medium power).
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ARG23456 anti-CD11b + CD11c antibody [OX-42] IHC-Fr image
Immunohistochemistry: Rat lymph node cryosection stained with ARG23456 anti-CD11b + CD11c antibody [OX-42], red in A and Mouse anti Rat CD8, green in B. C is the merged image with nuclei counter-stained in blue using DAPI. (High power).
