ARG65490
anti-CD11b antibody [MEM-174] (azide free)
anti-CD11b antibody [MEM-174] (azide free) for Flow cytometry,Immunoprecipitation and Human
MDSC Marker antibody; Myeloid-derived suppressor cell antibody
概述
| 产品描述 | Azide free Mouse Monoclonal antibody [MEM-174] recognizes CD11b |
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| 反应物种 | Hu |
| 应用 | FACS, IP |
| 特异性 | The clone MEM-174 recognizes CD11b antigen (Mac-1 alpha), a 165-170 kDa type I transmembrane protein mainly expressed on monocytes, granulocytes and NK-cells. The CD11b mediates neutrophil and monocyte interactions with stimulated endothelium. HLDA VI; WS Code BP 310 HLDA VI; WS Code M 18 |
| 宿主 | Mouse |
| 克隆 | Monoclonal |
| 克隆号 | MEM-174 |
| 同位型 | IgG2a |
| 靶点名称 | CD11b |
| 抗原物种 | Human |
| 抗原 | Human granulocytes |
| 偶联标记 | Un-conjugated |
| 別名 | MAC1A; CR3A; CR-3 alpha chain; Cell surface glycoprotein MAC-1 subunit alpha; Integrin alpha-M; MAC-1; CD11 antigen-like family member B; Leukocyte adhesion receptor MO1; MO1A; SLEB6; Neutrophil adherence receptor; CD antigen CD11b; CD11B |
应用说明
| 应用建议 |
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| 应用说明 | IP: Immunoprecipitation with the clone MEM-174 was successfuly performed using immuno-affinity sorbents (minicolumns). The final immunosorbent should contain 1-5 mg of antibody/1 ml of sorbent gel (e.g. agarose beads). It is recommended to stimulate positive control material by LPS 1-2 days before to achieve better expression of target antigen). * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
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| 阳性对照 | FACS: Peripheral Blood Lymphocytes IP: Granulocytes CD11b/CD18 transfectants |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Purification with Protein A. |
| 纯化说明 | 0.2 µm filter sterilized. |
| 纯度 | > 95% (by SDS-PAGE) |
| 缓冲液 | PBS (pH 7.4) |
| 浓度 | 1 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | ITGAM |
| 全名 | integrin, alpha M (complement component 3 receptor 3 subunit) |
| 背景介绍 | CD11b (integrin alpha M chain): Integrins are heterodimeric integral membrane proteins composed of an alpha chain and a beta chain. This I-domain containing alpha integrin combines with the beta 2 chain (ITGB2) to form a leukocyte-specific integrin referred to as macrophage receptor 1 ('Mac-1'), or inactivated-C3b (iC3b) receptor 3 ('CR3'). The alpha M beta 2 integrin is important in the adherence of neutrophils and monocytes to stimulated endothelium, and also in the phagocytosis of complement coated particles. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Mar 2009] |
| 生物功能 | CD11b: Integrin ITGAM/ITGB2 is implicated in various adhesive interactions of monocytes, macrophages and granulocytes as well as in mediating the uptake of complement-coated particles and pathogens (PubMed:9558116, PubMed:20008295). It is identical with CR-3, the receptor for the iC3b fragment of the third complement component. It probably recognizes the R-G-D peptide in C3b. Integrin ITGAM/ITGB2 is also a receptor for fibrinogen, factor X and ICAM1. It recognizes P1 and P2 peptides of fibrinogen gamma chain. Regulates neutrophil migration (PubMed:28807980). In association with beta subunit ITGB2/CD18, required for CD177-PRTN3-mediated activation of TNF primed neutrophils (PubMed:21193407). May regulate phagocytosis-induced apoptosis in extravasated neutrophils. May play a role in mast cell development. Required with TYROBP/DAP12 in microglia to control production of microglial superoxide ions which promote the neuronal apoptosis that occurs during brain development. [UniProt] |
| 产品亮点 | Related products: CD11b antibodies; CD11b Duos / Panels; Anti-Mouse IgG secondary antibodies; Related news: New antibody panels and duos for Tumor immune microenvironment Anti-SerpinB9 therapy, a new strategy for cancer therapy |
| 研究领域 | MDSC Marker antibody; Myeloid-derived suppressor cell antibody |
| 预测分子量 | 127 kDa |
检测图片 (1) Click the Picture to Zoom In
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ARG65490 anti-CD11b antibody [MEM-174] (azide free) IP image
Immunoprecipitation: PBMC lysate isolated from healthy donor was immunoprecipitated with ARG65490 anti-CD11b antibody [MEM-174] (azide free). Eluted immunoprecipitate was resolved on 7.5% SDS-PAGE and stained with Coomasie Blue.
