ARG43309
anti-CD137 antibody [4-1BB/3201]
anti-CD137 antibody [4-1BB/3201] for Flow cytometry,ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human
概述
| 产品描述 | Mouse Monoclonal antibody [4-1BB/3201] recognizes CD137 |
|---|---|
| 反应物种 | Hu |
| 应用 | FACS, ICC/IF, IHC-P, WB |
| 宿主 | Mouse |
| 克隆 | Monoclonal |
| 克隆号 | 4-1BB/3201 |
| 同位型 | IgG1, kappa |
| 靶点名称 | CD137 |
| 抗原物种 | Human |
| 抗原 | Recombinant protein fragment within aa. 19-188 of Human CD137. |
| 偶联标记 | Un-conjugated |
| 別名 | ILA; CD137; T-cell antigen 4-1BB homolog; CDw137; T-cell antigen ILA; 4-1BB; CD antigen CD137; Tumor necrosis factor receptor superfamily member 9; 4-1BB ligand receptor |
应用说明
| 应用建议 |
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|---|---|---|---|---|---|---|---|---|---|---|---|
| 应用说明 | IHC-P: Antigen Retrieval: Boil tissue section in 10 mM Tris with 1 mM EDTA (pH 9.0) for 10-20 min, followed by cooling at RT. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Purification with Protein G. |
| 缓冲液 | PBS, 0.05% Sodium azide and 0.1 mg/ml BSA. |
| 抗菌剂 | 0.05% Sodium azide |
| 稳定剂 | 0.1 mg/ml BSA |
| 浓度 | 0.2 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 |
Swiss-port # Q07011 Human Tumor necrosis factor receptor superfamily member 9 |
|---|---|
| 基因名称 | TNFRSF9 |
| 全名 | tumor necrosis factor receptor superfamily, member 9 |
| 背景介绍 | The protein encoded by this gene is a member of the TNF-receptor superfamily. This receptor contributes to the clonal expansion, survival, and development of T cells. It can also induce proliferation in peripheral monocytes, enhance T cell apoptosis induced by TCR/CD3 triggered activation, and regulate CD28 co-stimulation to promote Th1 cell responses. The expression of this receptor is induced by lymphocyte activation. TRAF adaptor proteins have been shown to bind to this receptor and transduce the signals leading to activation of NF-kappaB. [provided by RefSeq, Jul 2008] |
| 生物功能 | Receptor for TNFSF9/4-1BBL. Possibly active during T cell activation. [UniProt] |
| 细胞定位 | Membrane; Single-pass type I membrane protein. [UniProt] |
| 预测分子量 | 28 kDa |
检测图片 (7) Click the Picture to Zoom In
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ARG43309 anti-CD137 antibody [4-1BB/3201] ICC/IF image
Immunofluorescence: MeOH-fixed HEK293 cells stained with ARG43309 anti-CD137 antibody [4-1BB/3201] (green) and Reddot nuclear stain (red).
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ARG43309 anti-CD137 antibody [4-1BB/3201] IHC-P image
Immunohistochemistry: Formalin-fixed and paraffin-embedded Human spleen tissue. Antigen Retrieval: Boil tissue section in 10 mM Tris with 1 mM EDTA (pH 9.0) for 10-20 min, followed by cooling at RT. The tissue section was stained with ARG43309 anti-CD137 antibody [4-1BB/3201].
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ARG43309 anti-CD137 antibody [4-1BB/3201] WB image
Western blot: Human Kidney stained with ARG43309 anti-CD137 antibody [4-1BB/3201] .
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ARG43309 anti-CD137 antibody [4-1BB/3201] FACS image
Flow Cytometry: MeOH-fixed HEK293 cells stained with ARG43309 anti-CD137 antibody [4-1BB/3201] (blue) or isotype control (red).
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ARG43309 anti-CD137 antibody [4-1BB/3201] IHC-P image
Immunohistochemistry: Formalin-fixed and paraffin-embedded Human cerebellum tissue. Antigen Retrieval: Boil tissue section in 10 mM Tris with 1 mM EDTA (pH 9.0) for 10-20 min, followed by cooling at RT. The tissue section was stained with ARG43309 anti-CD137 antibody [4-1BB/3201].
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ARG43309 anti-CD137 antibody [4-1BB/3201] IHC-P image
Immunohistochemistry: Formalin-fixed and paraffin-embedded Human renal cell carcinoma. Antigen Retrieval: Boil tissue section in 10 mM Tris with 1 mM EDTA (pH 9.0) for 10-20 min, followed by cooling at RT. The tissue section was stained with ARG43309 anti-CD137 antibody [4-1BB/3201].
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ARG43309 anti-CD137 antibody [4-1BB/3201] IHC-P image
Immunohistochemistry: Formalin-fixed and paraffin-embedded Human renal cell carcinoma. Antigen Retrieval: Boil tissue section in 10 mM Tris with 1 mM EDTA (pH 9.0) for 10-20 min, followed by cooling at RT. The tissue section was stained with ARG43309 anti-CD137 antibody [4-1BB/3201].
