ARG42486

anti-CD15 antibody [FUT4/1478R]

anti-CD15 antibody [FUT4/1478R] for Flow cytometry,ICC/IF,IHC-Formalin-fixed paraffin-embedded sections and Human,Mouse,Rat

概述

产品描述 Recombinant Rabbit Monoclonal antibody [FUT4/1478R] recognizes CD15
反应物种 Hu, Ms, Rat
应用 FACS, ICC/IF, IHC-P
宿主 Rabbit
克隆 Monoclonal
克隆号 FUT4/1478R
同位型 IgG, kappa
靶点名称 CD15
抗原物种 Human
抗原 Purified Human neutrophils.
偶联标记 Un-conjugated
別名 LeX; CD15; ELFT; FCT3A; FUTIV; SSEA-1; FUC-TIV; Alpha-(1,3)-fucosyltransferase 4; EC 2.4.1.-; ELAM-1 ligand fucosyltransferase; Fucosyltransferase 4; Fucosyltransferase IV; Fuc-TIV; FucT-IV; Galactoside 3-L-fucosyltransferase

应用说明

应用建议
应用 推荐稀释比
FACS0.5 - 1 µg/10^6 cells
ICC/IF1 - 2 µg/ml
IHC-P0.5 - 1 µg/ml
应用说明 IHC-P: Antigen Retrieval: Steam tissue section in 10 mM Tris with 1 mM EDTA (pH 9.0) for 10-20 min.
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

属性

形式 Liquid
纯化 Purification with Protein A.
缓冲液 PBS, 0.05% Sodium azide and 0.1 mg/ml BSA.
抗菌剂 0.05% Sodium azide
稳定剂 0.1 mg/ml BSA
浓度 0.2 mg/ml
存放说明 For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
注意事项 For laboratory research only, not for drug, diagnostic or other use.

生物信息

数据库连接

GeneID: 14345 Mouse FUT4

GeneID: 2526 Human FUT4

Swiss-port # P22083 Human Alpha-(1,3)-fucosyltransferase 4

Swiss-port # Q11127 Mouse Alpha-(1,3)-fucosyltransferase 4

基因名称 FUT4
全名 fucosyltransferase 4 (alpha (1,3) fucosyltransferase, myeloid-specific)
背景介绍 The product of this gene transfers fucose to N-acetyllactosamine polysaccharides to generate fucosylated carbohydrate structures. It catalyzes the synthesis of the non-sialylated antigen, Lewis x (CD15). [provided by RefSeq, Jan 2009]
生物功能 May catalyze alpha-1,3 glycosidic linkages involved in the expression of Lewis X/SSEA-1 and VIM-2 antigens. [UniProt]
细胞定位 Golgi apparatus, Golgi stack membrane; Single-pass type II membrane protein. Note=Membrane-bound form in trans cisternae of Golgi. [UniProt]
预测分子量 59 kDa

检测图片 (3) Click the Picture to Zoom In

  • ARG42486 anti-CD15 antibody [FUT4/1478R] ICC/IF image

    Immunofluorescence: U937 cells stained with ARG42486 anti-CD15 antibody [FUT4/1478R] (green) and Reddot (red) for nuclear staining.

  • ARG42486 anti-CD15 antibody [FUT4/1478R] IHC-P image

    Immunohistochemistry: Formalin-fixed and paraffin-embedded Human Hodgkin's lymphoma tissue. Antigen Retrieval: Steam tissue section in 10 mM Tris with 1 mM EDTA (pH 9.0) for 10-20 min. The tissue section was stained with ARG42486 anti-CD15 antibody [FUT4/1478R].

  • ARG42486 anti-CD15 antibody [FUT4/1478R] FACS image

    Flow Cytometry: U937 cells stained with ARG42486 anti-CD15 antibody [FUT4/1478R] (blue) and isotype control (red).