ARG65419

anti-CD152 / CTLA4 antibody [BNI3]

Datasheet

订购信息

包装

价格 (USD)

100 μg

399

arigo in CHINA:
恩瑞科生物
☎ 400-886-0853,
0571-8708-1890

概述

产品描述	Mouse Monoclonal antibody [BNI3] recognizes CD152 / CTLA4
反应物种	Hu
应用	FACS, ICC/IF, IHC-Fr, IP
特异性	The clone BNI3 recognizes human CD152 / CTLA4, an approximately 45 kDa type I transmembrane protein serving as a negative regulator of T cell responses.
宿主	Mouse
克隆	Monoclonal
克隆号	BNI3
同位型	IgG2a
靶点名称	CD152 / CTLA4
抗原物种	Human
抗原	Human CD152-IgG heavy chain fusion protein
偶联标记	Un-conjugated
別名	GRD4; CTLA-4; CELIAC3; CD; Cytotoxic T-lymphocyte-associated antigen 4; CD152; GSE; CD antigen CD152; Cytotoxic T-lymphocyte protein 4; ALPS5; IDDM12

应用说明

应用建议

应用	推荐稀释比
FACS	2 - 5 µg/ml
ICC/IF	Assay-dependent
IHC-Fr	Assay-dependent
IP	Assay-dependent

应用说明

FACS: Intracellular staining.
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

属性

形式	Liquid
纯化	Purified from cell culture supernatant by protein-A affinity chromatography.
纯度	> 95% (by SDS-PAGE)
缓冲液	PBS (pH 7.4) and 15 mM Sodium azide
抗菌剂	15 mM Sodium azide
浓度	1 mg/ml
存放说明	For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
注意事项	For laboratory research only, not for drug, diagnostic or other use.

生物信息

数据库连接	GeneID: 1493 Human CTLA4 Swiss-port # P16410 Human Cytotoxic T-lymphocyte protein 4
基因名称	CTLA4
全名	cytotoxic T-lymphocyte-associated protein 4
背景介绍	CD152 / CTLA-4 is a homodimeric transmembrane protein similar to CD28 and binding the same ligands, i.e. CD80 (B7.1) and CD86 (B7.2), but with higher affinity. Unlike CD28 with important costimulating functions, CD152 acts as an important inhibitory receptor essential for modulation of the immune system. CD152 / CTLA-4 becomes transiently expressed on activated T cells and its malfunction can cause autoimmune diseases, such as insulin-dependent diabetes mellitus, Graves disease, Hashimoto thyroiditis, celiac disease, systemic lupus erythematosus, or thyroid-associated orbitopathy.
生物功能	Inhibitory receptor acting as a major negative regulator of T-cell responses. The affinity of CTLA4 for its natural B7 family ligands, CD80 and CD86, is considerably stronger than the affinity of their cognate stimulatory coreceptor CD28. [UniProt]
研究领域	Developmental Biology antibody; Immune System antibody
预测分子量	25 kDa
翻译后修饰	N-glycosylation is important for dimerization. Phosphorylation at Tyr-201 prevents binding to the AP-2 adapter complex, blocks endocytosis, and leads to retention of CTLA4 on the cell surface.

检测图片 (3) Click the Picture to Zoom In

ARG65419 anti-CD152 / CTLA4 antibody [BNI3] FACS image

Flow Cytometry: PHA stimulated human peripheral whole blood stained with ARG65419 anti-CD152 / CTLA4 antibody [BNI3] at 10 µg/ml dilution, followed by APC-conjugated Goat anti-Mouse antibody.
ARG65419 anti-CD152 / CTLA4 antibody [BNI3] FACS image

Flow Cytometry: PHA stimulated human lymphocytes stained with ARG65419 anti-CD152 / CTLA4 antibody [BNI3] at 10 µg/ml dilution, followed by APC-conjugated Goat anti-Mouse antibody. Cells were co-stained with ARG53820 anti-CD3 antibody [UCHT1] (PE) (20 µl reagent / 100 µl of peripheral whole blood).
ARG65419 anti-CD152 / CTLA4 antibody [BNI3] FACS image

Flow Cytometry: Separation of human CD152 positive CD3 positive lymphocytes (red-filled) from CD152 negative CD3 negative lymphocytes (black-dashed). Human peripheral whole blood stained with ARG65419 anti-CD152 / CTLA4 antibody [BNI3] at 10 µg/ml dilution, followed by APC-conjugated Goat anti-Mouse antibody.